Journal article
Circadian and light-driven regulation of rod dark adaptation
Scientific reports, Vol.5(1), pp.17616-17616
12/02/2015
DOI: 10.1038/srep17616
PMCID: PMC4667277
PMID: 26626567
Abstract
Continuous visual perception and the dark adaptation of vertebrate photoreceptors after bright light exposure require recycling of their visual chromophore through a series of reactions in the retinal pigmented epithelium (RPE visual cycle). Light-driven chromophore consumption by photoreceptors is greater in daytime vs. nighttime, suggesting that correspondingly higher activity of the visual cycle may be required. However, as rod photoreceptors are saturated in bright light, the continuous turnover of their chromophore by the visual cycle throughout the day would not contribute to vision. Whether the recycling of chromophore that drives rod dark adaptation is regulated by the circadian clock and light exposure is unknown. Here, we demonstrate that mouse rod dark adaptation is slower during the day or after light pre-exposure. This surprising daytime suppression of the RPE visual cycle was accompanied by light-driven reduction in expression of Rpe65, a key enzyme of the RPE visual cycle. Notably, only rods in melatonin-proficient mice were affected by this daily visual cycle modulation. Our results demonstrate that the circadian clock and light exposure regulate the recycling of chromophore in the RPE visual cycle. This daily melatonin-driven modulation of rod dark adaptation could potentially protect the retina from light-induced damage during the day.
Details
- Title: Subtitle
- Circadian and light-driven regulation of rod dark adaptation
- Creators
- Yunlu Xue - Washington University in St. LouisSusan Q. Shen - Washington University in St. LouisJoseph C. Corbo - Washington University in St. LouisVladimir J. Kefalov - Washington University in St. Louis
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.5(1), pp.17616-17616
- Publisher
- NATURE PORTFOLIO
- DOI
- 10.1038/srep17616
- PMID
- 26626567
- PMCID
- PMC4667277
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Number of pages
- 10
- Grant note
- NIH Roadmap for Medical Research; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA UL1RR024992 / ICTS/CTSA Grant from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) EY019312; EY021126; EY018826; HG006790; HG006346; 5T32EY013360; EY002687 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA P30 CA91842 / NCI Cancer Center Support Grant; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Research to Prevent Blindness; Research to Prevent Blindness (RPB)
- Language
- English
- Date published
- 12/02/2015
- Academic Unit
- Psychiatry; Iowa Neuroscience Institute
- Record Identifier
- 9984618635802771
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