Journal article
Circadian rhythm in intracellular Cl(-) activity of acutely dissociated neurons of suprachiasmatic nucleus
American Journal of Physiology: Cell Physiology, Vol.282(2), pp.C366-373
02/2002
DOI: 10.1152/ajpcell.00187.2000
PMID: 11788348
Abstract
A link between the circadian rhythm and the function of Cl(-)-permeable gamma-aminobutyric acid (GABA) type A (GABA(A)) receptors on suprachiasmatic nucleus (SCN) neurons was studied by measuring intracellular activity of Cl(-) (aCl) at different times during a circadian cycle in SCN neurons acutely dissociated from rat brains. To measure aCl, the voltage-clamp mode of the gramicidin-perforated patch-clamp technique was used, and reversal potential of GABA-induced currents (E(GABA)) was converted to aCl. Measured aCl was significantly higher at around noon (20.1 +/- 1.4 mM) than at three other time zones of a circadian cycle (means ranging from 11.6 to 14.3 mM). Chord conductance of GABA-induced currents showed no circadian changes, indicating a lack of circadian changes in the number or single-channel conductance of GABA(A) receptors. These results suggest that aCl participates in modulating GABA(A) receptor functions on SCN neurons during the circadian rhythm.
Details
- Title: Subtitle
- Circadian rhythm in intracellular Cl(-) activity of acutely dissociated neurons of suprachiasmatic nucleus
- Creators
- Masahiko Shimura - Department of Ophthalmology, Tohoku University School of Medicine, Sendai 980-8574, JapanNorio AkaikeNobutoshi Harata
- Resource Type
- Journal article
- Publication Details
- American Journal of Physiology: Cell Physiology, Vol.282(2), pp.C366-373
- Publisher
- United States
- DOI
- 10.1152/ajpcell.00187.2000
- PMID
- 11788348
- ISSN
- 0363-6143
- eISSN
- 1522-1563
- Language
- English
- Date published
- 02/2002
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984025431402771
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