Journal article
Circadian rhythms in the cell cycle and biomass composition of Neochloris oleoabundans under nitrogen limitation
Journal of biotechnology, Vol.187, pp.25-33
10/10/2014
DOI: 10.1016/j.jbiotec.2014.07.016
PMID: 25062660
Abstract
•Also under nitrogen limitation cell division was scheduled by the circadian clock.•Two subpopulations arose in the reactor dividing alternately every other day.•This phased cell division caused changes in biomass yield and composition.•Neutral lipids build up in the day, mostly in cells arrested in their cell cycle.
The circadian clock schedules processes in microalgae cells at suitable times in the day/night cycle. To gain knowledge about these biological time schedules, Neochloris oleoabundans was grown under constant light conditions and nitrogen limitation. Under these constant conditions, the only variable was the circadian clock. The results were compared to previous work done under nitrogen-replete conditions, in order to determine the effect of N-limitation on circadian rhythms in the cell cycle and biomass composition of N. oleoabundans.
The circadian clock was not affected by nitrogen-limitation, and cell division was timed in the natural night, despite of constant light conditions. However, because of nitrogen-limitation, not the entire population was able to divide every day. Two subpopulations were observed, which divided alternately every other day. This caused oscillations in biomass yield and composition. Starch and total fatty acids (TFA) were accumulated during the day. Also, fatty acid composition changed during the cell cycle. Neutral lipids were built up during the day, especially in cells that were arrested in their cell cycle (G2 and G3). These findings give insight in the influence of circadian rhythms on the cell cycle and biomass composition.
Details
- Title: Subtitle
- Circadian rhythms in the cell cycle and biomass composition of Neochloris oleoabundans under nitrogen limitation
- Creators
- Lenneke de Winter - Wageningen University & ResearchLutz W. Schepers - Wageningen University & ResearchMaria Cuaresma - Bioprocess Engineering, AlgaePARC, Wageningen University, www.algaePARC.com, PO Box 8129, 6700 EV Wageningen, The NetherlandsMaria J. Barbosa - Wageningen UR Food and Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The NetherlandsDirk E. Martens - Wageningen University & ResearchRené H. Wijffels - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Journal of biotechnology, Vol.187, pp.25-33
- DOI
- 10.1016/j.jbiotec.2014.07.016
- PMID
- 25062660
- NLM abbreviation
- J Biotechnol
- ISSN
- 0168-1656
- eISSN
- 1873-4863
- Publisher
- Elsevier B.V
- Grant note
- name: Dutch ministry of Economic Affairs
- Language
- English
- Date published
- 10/10/2014
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984397919302771
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