Circulating cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) in oncology: from technological advances to clinical applications

Edoardo Francini; Sara Bleve; Giuseppe Nicolo Fanelli; Mara Serena Serafini; Filippo Pederzoli; Alessandra Ferri; Erika Minonne; Jacopo Venturini; Silvia Rodrigues; Hubert Pakula; Cristian Lolli; Massimo Cristofanilli; Pier Vitale Nuzzo

doi:10.1038/s41698-026-01507-w

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Circulating cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) in oncology: from technological advances to clinical applications

Journal article

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Circulating cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) in oncology: from technological advances to clinical applications

Edoardo Francini, Sara Bleve, Giuseppe Nicolo Fanelli, Mara Serena Serafini, Filippo Pederzoli, Alessandra Ferri, Erika Minonne, Jacopo Venturini, Silvia Rodrigues, Hubert Pakula, …

NPJ precision oncology, Vol.10(1), p.318

05/25/2026

DOI: 10.1038/s41698-026-01507-w

PMID: 42185453

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Abstract

Circulating cell-free DNA (cfDNA) methylation profiling enables minimally invasive cancer detection and monitoring. Among available methods, cfMeDIP-seq is a sensitive, scalable, bisulfite-free approach suitable for low-input cfDNA. This review summarizes its principles, comparative technologies, and clinical applications, including early detection, tumor classification, and minimal residual disease monitoring. Despite promising performance, challenges remain in standardization and validation, while emerging multi-omic integrations may further enhance its role in precision oncology.

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Title: Subtitle: Circulating cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) in oncology: from technological advances to clinical applications
Creators: Edoardo Francini - University of Florence
Sara Bleve - Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
Giuseppe Nicolo Fanelli - University of Pisa
Mara Serena Serafini - Cornell University
Filippo Pederzoli - Cornell University
Alessandra Ferri - Cornell University
Erika Minonne - Cornell University
Jacopo Venturini - Sarah Cannon Research Institute
Silvia Rodrigues - Cornell University
Hubert Pakula - Cornell University
Cristian Lolli - Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
Massimo Cristofanilli - Cornell University
Pier Vitale Nuzzo - Cornell University
Resource Type: Journal article
Publication Details: NPJ precision oncology, Vol.10(1), p.318
DOI: 10.1038/s41698-026-01507-w
PMID: 42185453
ISSN: 2397-768X
eISSN: 2397-768X
Publisher: Nature Publishing Group
Language: English
Date published: 05/25/2026
Academic Unit: Internal Medicine
Record Identifier: 9985221147702771

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