ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls

Jessika Feliciano; Shifen Xu; Xiyuan Guan; Hans-Joachim Lehmler; Leonidas Bachas; Sylvia Daunert

doi:10.1007/s00216-006-0505-3

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ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls

Journal article

Peer reviewed

ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls

Jessika Feliciano, Shifen Xu, Xiyuan Guan, Hans-Joachim Lehmler, Leonidas Bachas and Sylvia Daunert

Analytical and bioanalytical chemistry, Vol.385(5), pp.807-813

07/2006

DOI: 10.1007/s00216-006-0505-3

PMID: 16791559

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Abstract

Polychlorinated biphenyls (PCBs) are a group of organic pollutants that are persistent when released into the environment. Among the metabolites of PCBs, dihydroxylated PCBs are also considered as toxic compounds. Various studies have shown that dihydroxylated PCBs affect the reproductive, immune, nervous, and endocrine systems. Detection of these chemicals in environmental and biological samples could provide first-hand information about their levels and lead to a better understanding of their role in toxicity. To that end, we developed a sensing system for the detection of dihydroxylated PCBs based on the clc operon. The Pseudomonas putida clc operon encodes a catabolic pathway for degradation of chlorocatechols, which are major metabolites of a large number of chlorinated compounds. In P. putida, the expression of these genes is regulated by a protein encoded by the gene clcR located upstream from the clcABD genes. We demonstrate here for the first time that dihydroxy PCBs can also induce the clc operon. Our sensing system employs P. putida bacteria harboring a plasmid in which the reporter gene, lacZ, is under the control of the regulatory protein ClcR. Consequently, when exposed to dihydroxy PCBs, the bacteria express β-galactosidase in an amount related to the concentration of the corresponding dihydroxy PCB. Various dihydroxylated PCBs, differing in the number and position of chlorines and in the position of hydroxyls, were tested for their ability to induce expression of β-galactosidase. Detection limits as low as 1×10−6 mol L−1 were obtained for various dihydroxylated PCBs.

Chemistry

Analytical Chemistry

Food Science

Reporter gene

Monitoring/Environmental Analysis/Environmental Ecotoxicology

Clc operon

Physical Chemistry

β -galactosidase

Inorganic Chemistry

PCB

Whole-cell biosensor

Details

Title: Subtitle: ClcR-based biosensing system in the detection of cis-dihydroxylated (chloro-)biphenyls
Creators: Jessika Feliciano - Department of Chemistry University of Kentucky Lexington KY 40506-0055 USA
Shifen Xu - Department of Chemistry University of Kentucky Lexington KY 40506-0055 USA
Xiyuan Guan - Department of Chemistry University of Kentucky Lexington KY 40506-0055 USA
Hans-Joachim Lehmler - Department of Occupational and Environmental Health University of Iowa Iowa IA 52242-5000 USA
Leonidas Bachas - Department of Chemistry University of Kentucky Lexington KY 40506-0055 USA
Sylvia Daunert - Department of Chemistry University of Kentucky Lexington KY 40506-0055 USA
Resource Type: Journal article
Publication Details: Analytical and bioanalytical chemistry, Vol.385(5), pp.807-813
DOI: 10.1007/s00216-006-0505-3
PMID: 16791559
NLM abbreviation: Anal Bioanal Chem
ISSN: 1618-2642
eISSN: 1618-2650
Publisher: Springer-Verlag; Berlin/Heidelberg
Language: English
Date published: 07/2006
Academic Unit: Occupational and Environmental Health; Iowa Neuroscience Institute
Record Identifier: 9984001084502771

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