Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast

Nadia Vadaie; Heather Dionne; Darowan S Akajagbor; Seth R Nickerson; Damian J Krysan; Paul J Cullen

doi:10.1083/jcb.200704079

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Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast

Journal article

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Peer reviewed

Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast

Nadia Vadaie, Heather Dionne, Darowan S Akajagbor, Seth R Nickerson, Damian J Krysan and Paul J Cullen

The Journal of cell biology, Vol.181(7), pp.1073-1081

06/30/2008

DOI: 10.1083/jcb.200704079

PMCID: PMC2442203

PMID: 18591427

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Abstract

Signaling mucins are cell adhesion molecules that activate RAS/RHO guanosine triphosphatases and their effector mitogen-activated protein kinase (MAPK) pathways. We found that the Saccharomyces cerevisiae mucin Msb2p, which functions at the head of the Cdc42p-dependent MAPK pathway that controls filamentous growth, is processed into secreted and cell-associated forms. Cleavage of the extracellular inhibitory domain of Msb2p by the aspartyl protease Yps1p generated the active form of the protein by a mechanism incorporating cellular nutritional status. Activated Msb2p functioned through the tetraspan protein Sho1p to induce MAPK activation as well as cell polarization, which involved the Cdc42p guanine nucleotide exchange factor Cdc24p. We postulate that cleavage-dependent activation is a general feature of signaling mucins, which brings to light a novel regulatory aspect of this class of signaling adhesion molecule.

Protein Structure, Tertiary

Signal Transduction

Aspartic Acid Endopeptidases - biosynthesis

Enzyme Induction

Intracellular Signaling Peptides and Proteins

Saccharomyces cerevisiae Proteins - biosynthesis

GTPase-Activating Proteins - chemistry

GTPase-Activating Proteins - metabolism

MAP Kinase Signaling System

Saccharomyces cerevisiae - cytology

Peptides - metabolism

Guanine Nucleotide Exchange Factors - metabolism

Aspartic Acid Endopeptidases - metabolism

Mucins - metabolism

Saccharomyces cerevisiae Proteins - metabolism

Protein Binding

Saccharomyces cerevisiae - enzymology

Protein Processing, Post-Translational

Enzyme Activation

Mitogen-Activated Protein Kinases - metabolism

Saccharomyces cerevisiae Proteins - chemistry

Details

Title: Subtitle: Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast
Creators: Nadia Vadaie - Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA
Heather Dionne
Darowan S Akajagbor
Seth R Nickerson
Damian J Krysan
Paul J Cullen
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.181(7), pp.1073-1081
DOI: 10.1083/jcb.200704079
PMID: 18591427
PMCID: PMC2442203
ISSN: 0021-9525
eISSN: 1540-8140
Grant note: GM 0535393T / NIGMS NIH HHS K08 AI062978 / NIAID NIH HHS 1R03DE018425-01 / NIDCR NIH HHS R03 DE018425 / NIDCR NIH HHS K08AI062978 / NIAID NIH HHS
Language: English
Date published: 06/30/2008
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984093373702771

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