Journal article
Clinical evaluation of a dried commercially prepared microdilution panel for antifungal susceptibility testing of five antifungal agents against Candida spp. and Cryptococcus neoformans
Diagnostic microbiology and infectious disease, Vol.50(2), pp.113-117
2004
DOI: 10.1016/j.diagmicrobio.2004.06.013
PMID: 15474320
Abstract
A commercially prepared dried-broth microdilution panel (Sensititre, TREK Diagnostic Systems, Cleveland, OH) was compared with a reference frozen-broth microdilution panel for antifungal susceptibility testing of 728 clinical isolates of
Candida spp. and 98 clinical isolates of
Cryptococcus neoformans. The antifungal agents tested were amphotericin B, fluconazole, 5-fluorocytosine (5FC), itraconazole, and voriconazole. Microdilution testing was performed according to NCCLS recommendations. Minimum inhibitory concentration (MIC) endpoints were read visually after 48 hours of incubation (72 hours for
C. neoformans) and were assessed independently for each microdilution panel. Discrepancies among MIC endpoints of no more than 2 log
2 dilutions were used to calculate the percentage of agreement. Overall levels of agreement between the study and reference panels were 98% for
Candida spp. and 93% for
C. neoformans. The agreement for each antifungal agent ranged from 96.6% for voriconazole to 99.4% for amphotericin B. The TREK dried microdilution panel appears to be a viable alternative to frozen-broth microdilution panels for testing of
Candida spp. and
C. neoformans.
Details
- Title: Subtitle
- Clinical evaluation of a dried commercially prepared microdilution panel for antifungal susceptibility testing of five antifungal agents against Candida spp. and Cryptococcus neoformans
- Creators
- M.A Pfaller - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, and College of Public Health, University of Iowa, Iowa City, IA, USAL Boyken - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, and College of Public Health, University of Iowa, Iowa City, IA, USAR J Hollis - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, and College of Public Health, University of Iowa, Iowa City, IA, USAS.A Messer - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, and College of Public Health, University of Iowa, Iowa City, IA, USAS Tendolkar - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, and College of Public Health, University of Iowa, Iowa City, IA, USAD.J Diekema - Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, and College of Public Health, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.50(2), pp.113-117
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.diagmicrobio.2004.06.013
- PMID
- 15474320
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 2004
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9983986365102771
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