Clinical evaluation of the Sensititre YeastOne colorimetric antifungal panel for antifungal susceptibility testing of the echinocandins anidulafungin, caspofungin, and micafungin

M A Pfaller; V Chaturvedi; D J Diekema; M A Ghannoum; N M Holliday; S B Killian; C C Knapp; S A Messer; A Miskov; R Ramani

doi:10.1128/JCM.00493-08

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Clinical evaluation of the Sensititre YeastOne colorimetric antifungal panel for antifungal susceptibility testing of the echinocandins anidulafungin, caspofungin, and micafungin

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Clinical evaluation of the Sensititre YeastOne colorimetric antifungal panel for antifungal susceptibility testing of the echinocandins anidulafungin, caspofungin, and micafungin

M A Pfaller, V Chaturvedi, D J Diekema, M A Ghannoum, N M Holliday, S B Killian, C C Knapp, S A Messer, A Miskov and R Ramani

Journal of clinical microbiology, Vol.46(7), pp.2155-2159

07/2008

DOI: 10.1128/JCM.00493-08

PMCID: PMC2446941

PMID: 18463213

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Abstract

A commercially prepared, dried colorimetric microdilution panel (Sensititre YeastOne Trek Diagnostic Systems, Cleveland, OH) was compared in three different laboratories with the Clinical and Laboratory Standards Institute (CLSI) reference microdilution method by testing 2 quality control strains, 25 reproducibility strains, and 404 isolates of Candida spp. against anidulafungin, caspofungin, and micafungin. Reference MIC endpoints and YeastOne colorimetric endpoints were read after 24 h of incubation. YeastOne endpoints were determined to be the lowest concentration at which the color in the well changed from red (positive, indicating growth) to blue (negative, indicating no growth). Excellent essential agreement (within 2 dilutions) between the reference and colorimetric MICs was observed. Overall agreement was 100% for all three agents. Categorical agreement ranged from 99.3% (anidulafungin) to 100% (caspofungin, micafungin) and interlaboratory reproducibility was 99%. The YeastOne colorimetric method appears to be comparable to the CLSI reference method for testing the susceptibility of Candida spp. to the echinocandins anidulafungin, caspofungin, and micafungin.

Antifungal Agents - pharmacology

Reference Standards

Reproducibility of Results

Microbial Sensitivity Tests - standards

Lipoproteins - pharmacology

Humans

Sensitivity and Specificity

Candida - drug effects

Candidiasis - microbiology

Lipopeptides

Microbial Sensitivity Tests - methods

Echinocandins - pharmacology

Details

Title: Subtitle: Clinical evaluation of the Sensititre YeastOne colorimetric antifungal panel for antifungal susceptibility testing of the echinocandins anidulafungin, caspofungin, and micafungin
Creators: M A Pfaller - Department of Pathology, Medical Microbiology Division, University of Iowa College of Medicine, Iowa City, IA 52242, USA. michael-pfaller@uiowa.edu
V Chaturvedi
D J Diekema
M A Ghannoum
N M Holliday
S B Killian
C C Knapp
S A Messer
A Miskov
R Ramani
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.46(7), pp.2155-2159
DOI: 10.1128/JCM.00493-08
PMID: 18463213
PMCID: PMC2446941
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 07/2008
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986259102771

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