Clinical isolates of GB virus type C vary in their ability to persist and replicate in peripheral blood mononuclear cell cultures

Sarah L George; Jinhua Xiang; Jack T Stapleton

doi:10.1016/S0042-6822(03)00585-3

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Clinical isolates of GB virus type C vary in their ability to persist and replicate in peripheral blood mononuclear cell cultures

Journal article

Open access

Peer reviewed

Clinical isolates of GB virus type C vary in their ability to persist and replicate in peripheral blood mononuclear cell cultures

Sarah L George, Jinhua Xiang and Jack T Stapleton

Virology (New York, N.Y.), Vol.316(2), pp.191-201

2003

DOI: 10.1016/S0042-6822(03)00585-3

PMID: 14644602

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Abstract

GB virus C/hepatitis G virus (GBV-C) replication in vitro is inefficient and inconsistent. In this study, clinical isolates of GBV-C were evaluated using peripheral blood mononuclear cell (PBMC) based culture methods. Isolates varied consistently in their ability to persistently replicate, and yield increased in cells grown without PHA/IL-2 stimulation. The deduced polyprotein sequence of an isolate that replicated well was determined (GenBank AY196904) and compared to 20 full-length GBV-C sequences. Fourteen of the 16 unique amino acid polymorphisms identified were in the coding regions for nonstructural proteins associated with interferon resistance and RNA replication. These data indicate that clinical GBV-C isolates vary in their ability to persist in culture, do not require PHA/IL-2 stimulation, and that sequence variability in key regulatory regions may affect growth in PBMC cultures. Since GBV-C appears to inhibit HIV replication in a coinfection model, these studies should facilitate determination of the mechanism of this interaction.

Hepatitis G

GB virus

In vitro replication

Polyprotein

Details

Title: Subtitle: Clinical isolates of GB virus type C vary in their ability to persist and replicate in peripheral blood mononuclear cell cultures
Creators: Sarah L George - Departments of Internal Medicine and Research, Iowa City VA Medical Center, University of Iowa, and the Helen C. Levitt Center for Viral Pathogenesis, Iowa City, IA 52242, USA
Jinhua Xiang - Departments of Internal Medicine and Research, Iowa City VA Medical Center, University of Iowa, and the Helen C. Levitt Center for Viral Pathogenesis, Iowa City, IA 52242, USA
Jack T Stapleton - Departments of Internal Medicine and Research, Iowa City VA Medical Center, University of Iowa, and the Helen C. Levitt Center for Viral Pathogenesis, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.316(2), pp.191-201
DOI: 10.1016/S0042-6822(03)00585-3
PMID: 14644602
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Language: English
Date published: 2003
Academic Unit: Microbiology and Immunology; Infectious Diseases; Internal Medicine
Record Identifier: 9984094611102771

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