Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Brian W Biegler; Shirley X Yan; Sterling B Ortega; Deepani K Tennakoon; Michael K Racke; Nitin J Karandikar

doi:10.1016/j.jneuroim.2011.02.001

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Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Journal article

Open access

Peer reviewed

Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis

Brian W Biegler, Shirley X Yan, Sterling B Ortega, Deepani K Tennakoon, Michael K Racke and Nitin J Karandikar

Journal of neuroimmunology, Vol.234(1-2), pp.131-140

05/2011

DOI: 10.1016/j.jneuroim.2011.02.001

PMCID: PMC3092854

PMID: 21397340

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https://www.ncbi.nlm.nih.gov/pmc/articles/3092854View

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Abstract

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.

Multiple sclerosis

TCR

CD4

Myelin

CDR3

CD8

Details

Title: Subtitle: Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis
Creators: Brian W Biegler - Department of Pathology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9072, USA
Shirley X Yan - Department of Pathology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9072, USA
Sterling B Ortega - Department of Pathology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9072, USA
Deepani K Tennakoon - Department of Pathology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9072, USA
Michael K Racke - Department of Neurology, Ohio State University Medical Center, Columbus, OH 43210, USA
Nitin J Karandikar - Department of Pathology, UT Southwestern Medical Center, 6000 Harry Hines Blvd., Dallas, TX 75390-9072, USA
Resource Type: Journal article
Publication Details: Journal of neuroimmunology, Vol.234(1-2), pp.131-140
DOI: 10.1016/j.jneuroim.2011.02.001
PMID: 21397340
PMCID: PMC3092854
NLM abbreviation: J Neuroimmunol
ISSN: 0165-5728
eISSN: 1872-8421
Publisher: Elsevier B.V
Grant note: name: USPHS NIH, award: AI053439, AI065463, AI079272, NS037513; DOI: 10.13039/100000890, name: National MS Society, award: JF2118-A-2
Language: English
Date published: 05/2011
Academic Unit: Pathology
Record Identifier: 9984047800102771

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