Journal article
Cloning Small RNAs
Rna Interference Techniques, Vol.58, pp.77-90
01/01/2011
DOI: 10.1007/978-1-61779-114-7_5
Abstract
Small, noncoding RNAs have proven to be powerful and ubiquitous agents of gene regulation in eukaryotic genomes. Since their initial discovery, several methods have been developed for directly cloning and sequencing these tiny RNA species. These cloning methods are presented along with a discussion of ways to enhance cloning efficiency and success. In addition, major advances in massively parallel, next-generation sequencing methods are presented as they impact small RNA studies. Finally, results of the application of one of the extant in vitro cloning methods to a marsupial mammal model are presented.
Details
- Title: Subtitle
- Cloning Small RNAs
- Creators
- Eric J. Devor - University of IowaLingyan Huang
- Resource Type
- Journal article
- Publication Details
- Rna Interference Techniques, Vol.58, pp.77-90
- DOI
- 10.1007/978-1-61779-114-7_5
- ISSN
- 0893-2336
- Copyright
- © Springer Science+Business Media, LLC 2011
- Language
- English
- Date published
- 01/01/2011
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983557777302771
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