Journal article
Cloning and characterization of the gene, speC, for pyrogenic exotoxin type C from Streptococcus pyogenes
Molecular & general genetics, Vol.212(1), pp.66-70
04/1988
DOI: 10.1007/BF00322445
PMID: 2836707
Abstract
The structural gene of streptococcal pyrogenic exotoxin type C (SPE C) was cloned from the chromosome of Streptococcus pyogenes strain T18P into Escherichia coli using pBR328 as the vector plasmid. Subcloning enabled the localization of the gene (speC) to a 1.7 kb fragment. Partially purified E. coli-derived SPE C and purified streptococcal-derived SPE C, were shown to have the same molecular weight (23,800) and biological activities. A DNA probe, prepared from cloned speC, cross-hybridized with the structural genes of SPE A and SPE B indicating relatedness at the nucleotide level. The speC-derived probe also hybridized to a fragment of CS112 bacteriophage DNA containing the phage attachment site.
Details
- Title: Subtitle
- Cloning and characterization of the gene, speC, for pyrogenic exotoxin type C from Streptococcus pyogenes
- Creators
- Stephen C GoshornGregory A BohachPatrick M Schlievert
- Resource Type
- Journal article
- Publication Details
- Molecular & general genetics, Vol.212(1), pp.66-70
- DOI
- 10.1007/BF00322445
- PMID
- 2836707
- ISSN
- 0026-8925
- eISSN
- 1432-1874
- Language
- English
- Date published
- 04/1988
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001222702771
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