Cloning and characterization of the segment polarity gene cubitus interruptus Dominant of Drosophila

T V Orenic; D C Slusarski; K L Kroll; R A Holmgren

doi:10.1101/gad.4.6.1053

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Cloning and characterization of the segment polarity gene cubitus interruptus Dominant of Drosophila

Journal article

Open access

Peer reviewed

Cloning and characterization of the segment polarity gene cubitus interruptus Dominant of Drosophila

T V Orenic, D C Slusarski, K L Kroll and R A Holmgren

Genes & development, Vol.4(6), pp.1053-1067

06/1990

DOI: 10.1101/gad.4.6.1053

PMID: 2166702

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Abstract

The segment polarity mutation, cubitus interruptus Dominant (ciD), of Drosophila melanogaster causes defects in the posterior half of every embryonic segment. We cloned sequences from the ciD region on the proximal fourth chromosome by "tagging" the gene with the transposable element P. Genetic and molecular evidence indicates that the P-element insertions, which all occurred within the same restriction fragment, are in 5'-regulatory regions of the ciD gene within 3 kb of the first exon of its transcript. The putative ciD transcript was identified on the basis of its absence in homozygous ciD embryos. Its spatial pattern of expression during development is unusual in that, unlike most other segmentation genes, it exhibits uniform expression throughout cellular blastoderm and gastrulation and does not resolve into a periodic pattern until the end of the fast phase of germ-band elongation when it is present in 15 broad segmentally repeating stripes along the anterior-posterior axis of the embryo. Registration of the ciD stripes of expression relative to the stripes of other segment polarity genes shows that ciD is expressed in the anterior three-quarters of every segment. This registration does not correlate with the pattern defects observed in ciD mutants. Sequence analysis indicates that the protein encoded by the ciD transcript contains a domain of five tandem amino acid repeats that have sequence similarity to the zinc-finger repeats of the Xenopus transcription factor TFIIIA and that share the highest degree of identity with the human zinc-finger protein GLI, which has been found to be amplified in several human glioblastomas.

Amino Acid Sequence

Drosophila melanogaster - embryology

Gene Expression

Zinc - metabolism

Molecular Sequence Data

DNA-Binding Proteins - genetics

Drosophila melanogaster - genetics

Restriction Mapping

DNA Transposable Elements

DNA-Binding Proteins - metabolism

Blotting, Northern

Metalloproteins - metabolism

Animals

Genes, Dominant

Base Sequence

Cloning, Molecular

Metalloproteins - genetics

Details

Title: Subtitle: Cloning and characterization of the segment polarity gene cubitus interruptus Dominant of Drosophila
Creators: T V Orenic - Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, Illinois 60208
D C Slusarski
K L Kroll
R A Holmgren
Resource Type: Journal article
Publication Details: Genes & development, Vol.4(6), pp.1053-1067
Publisher: United States
DOI: 10.1101/gad.4.6.1053
PMID: 2166702
ISSN: 0890-9369
eISSN: 1549-5477
Language: English
Date published: 06/1990
Academic Unit: Iowa Neuroscience Institute; Biology; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9983992048202771

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