Journal article
Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis
Molecular & general genetics, Vol.208(1-2), pp.84-87
06/1987
DOI: 10.1007/BF00330426
PMID: 3112526
Abstract
The genes encoding streptococcal pyrogenic exotoxin type A (SPE A) and staphylococcal toxic shock syndrome toxin-1 (TSST-1) were stably cloned and expressed in Bacillus subtilis. In the non-pathogenic Bacillus background, the recombinant speA clone expressed 32-fold more SPE A than the native streptococcus, and similarly, the recombinant plasmid harboring tst expressed 4-fold more TSST-1 in Bacillus than in the native Staphylococcus aureus. The Bacillus-derived products were secreted into the culture fluid, were resistant to proteolytic degradation and their biological activities mimicked native preparations.
Details
- Title: Subtitle
- Cloning and expression of streptococcal pyrogenic exotoxin A and staphylococcal toxic shock syndrome toxin-1 in Bacillus subtilis
- Creators
- B N KreiswirthJ P HandleyP M SchlievertR P Novick
- Resource Type
- Journal article
- Publication Details
- Molecular & general genetics, Vol.208(1-2), pp.84-87
- DOI
- 10.1007/BF00330426
- PMID
- 3112526
- NLM abbreviation
- Mol Gen Genet
- ISSN
- 0026-8925
- eISSN
- 1432-1874
- Publisher
- Germany
- Grant note
- AI22159 / NIAID NIH HHS
- Language
- English
- Date published
- 06/1987
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001154702771
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