Journal article
Cloning and expression of the beta- and gamma-subunits of the human epithelial sodium channel
American Journal of Physiology: Cell Physiology, Vol.268(5), pp.C1157-C1163
05/01/1995
DOI: 10.1152/ajpcell.1995.268.5.C1157
PMID: 7762608
Abstract
Amiloride-sensitive Na+ channels are an important component of the Na+ reabsorption pathway in a number of epithelia. Here we report the cloning and characterization of cDNAs encoding two subunits of the human kidney epithelial Na+ channel (beta- and gamma-hENaC). Their predicted amino acid sequences were highly homologous (83-85% identical) to the corresponding subunits reported from rat colon (beta- and gamma-rENaC). Both beta- and gamma-hENaC mapped to human chromosome 16. Northern blot analysis showed high expression of beta- and gamma-hENaC in kidney and lung and differential expression of the three subunits in other tissues. Coexpression of beta- and gamma-hENaC with alpha-hENaC in Xenopus oocytes produced Na+ channels with high selectivity for Na+ and high sensitivity to amiloride. In addition, human subunits were able to substitute for the corresponding rat subunits in forming functional Na+ channels, suggesting conservation of function and suggesting that differences in sequence do not disrupt interactions between subunits. These results suggest that human alpha-, beta-, and gamma-ENaC together form Na+ channels with properties that are similar to those observed in epithelia, and will allow further investigation into the role that these channels may play in human disease.
Details
- Title: Subtitle
- Cloning and expression of the beta- and gamma-subunits of the human epithelial sodium channel
- Creators
- F. J McDonald - Department of Internal Medicine, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242, USAM. P Price - Department of Internal Medicine, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242, USAP. M Snyder - Department of Internal Medicine, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242, USAM. J Welsh - Department of Internal Medicine, Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- American Journal of Physiology: Cell Physiology, Vol.268(5), pp.C1157-C1163
- DOI
- 10.1152/ajpcell.1995.268.5.C1157
- PMID
- 7762608
- ISSN
- 0363-6143
- eISSN
- 1522-1563
- Language
- English
- Date published
- 05/01/1995
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Medicine Administration; Internal Medicine
- Record Identifier
- 9984020738502771
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