Journal article
Cloning and identification of microRNAs in bovine alveolar macrophages
Molecular and cellular biochemistry, Vol.332(1-2), pp.9-16
12/2009
DOI: 10.1007/s11010-009-0168-4
PMID: 19507003
Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs found in both animal and plant cells. These approximately 19-26 nucleotide (nt) single-stranded RNAs play role in regulating gene/protein expression by either directly binding to mRNAs and inducing degradation of their target or by translational inhibition of protein expression. In this report, we described the cloning and identification of 22 microRNAs from bovine alveolar macrophage (AM). Eleven of the 22 miRNAs were novel and have not been previously identified in any species, while eight previously unidentified bovine miRNAs were identical to known ortholog miRNAs from human and/or rat (Bta-miR-141, Bta-miR-187, Bta-miR-191, Bta-miR-448, Bta-miR-589, Bta-miR-873, Bta-miR-463, and Bta-miR-562). These results add to the growing database of new miRNAs and suggest new biological functions for miRNAs in AMs. Specially, our data implicates miRNA regulation of antimicrobial targets in alveolar macrophages of bovine lung.
Details
- Title: Subtitle
- Cloning and identification of microRNAs in bovine alveolar macrophages
- Creators
- Guangxian Xu - College of Life Science, Ningxia University, Xixia District, Yinchuan, Ningxia, 750021, ChinaYan ZhangHao JiaJuan LiXiaoming LiuJohn F EngelhardtYujiong Wang
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biochemistry, Vol.332(1-2), pp.9-16
- Publisher
- Netherlands
- DOI
- 10.1007/s11010-009-0168-4
- PMID
- 19507003
- ISSN
- 0300-8177
- eISSN
- 1573-4919
- Grant note
- P30 DK054759 / NIDDK NIH HHS R37 DK047967 / NIDDK NIH HHS
- Language
- English
- Date published
- 12/2009
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Epidemiology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025347302771
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