Journal article
Cloning and nitrate induction of nitrate reductase mRNA
Proceedings of the National Academy of Sciences - PNAS, Vol.83(18), pp.6825-6828
09/1986
DOI: 10.1073/pnas.83.18.6825
PMCID: PMC386602
PMID: 16593758
Abstract
Nitrate is the major source of nitrogen taken from the soil by higher plants but requires reduction to ammonia prior to incorporation into amino acids. The first enzyme in the reducing pathway is a nitrate-inducible enzyme, nitrate reductase (EC 1.6.6.1). A specific polyclonal antiserum raised against purified barley nitrate reductase has been used to immunoprecipitate
in vivo
labeled protein and
in vitro
translation products, demonstrating that nitrate induction increases nitrate reductase protein and translatable mRNA. A partial cDNA clone for barley nitrate reductase has been isolated and identified by hybrid-selected translation. RNA blot-hybridization analysis shows that nitrate induction also causes a marked increase in the steady-state level of nitrate reductase mRNA.
Details
- Title: Subtitle
- Cloning and nitrate induction of nitrate reductase mRNA
- Creators
- Chi-Lien Cheng - Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 Department of Genetics, Harvard Medical School, Boston, MA 02114 Department of Agronomy and Soils, and Program in Genetics and Cell Biology, Washington State University, Pullman, WA 99164Julia Dewdney - Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 Department of Genetics, Harvard Medical School, Boston, MA 02114 Department of Agronomy and Soils, and Program in Genetics and Cell Biology, Washington State University, Pullman, WA 99164Andris Kleinhofs - Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 Department of Genetics, Harvard Medical School, Boston, MA 02114 Department of Agronomy and Soils, and Program in Genetics and Cell Biology, Washington State University, Pullman, WA 99164Howard M Goodman - Department of Molecular Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114 Department of Genetics, Harvard Medical School, Boston, MA 02114 Department of Agronomy and Soils, and Program in Genetics and Cell Biology, Washington State University, Pullman, WA 99164
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.83(18), pp.6825-6828
- DOI
- 10.1073/pnas.83.18.6825
- PMID
- 16593758
- PMCID
- PMC386602
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 09/1986
- Academic Unit
- Biology
- Record Identifier
- 9984217543002771
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