Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter

P W Hewett; E L Daft; J C Murray

doi:10.1006/bbrc.1998.9690

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Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter

Journal article

Peer reviewed

Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter

P W Hewett, E L Daft and J C Murray

Biochemical and biophysical research communications, Vol.252(3), pp.546-551

11/27/1998

DOI: 10.1006/bbrc.1998.9690

PMID: 9837743

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Abstract

The Tie-2 receptor plays a key role in vascular development, although little is known about the factors controlling its expression. Here we report the first cloning and characterisation of the 5' regulatory region of human tie-2. Multiple transcription start sites were identified between -414 and -265 bp upstream of the start codon using 5' RACE, fluorescent primer extension, and RNase protection assays. The human tie-2 promoter contains several transcription factor-binding sequences including ets, SP-1, AP-1, and GATA-1, but there are no canonical TATA or CCAAT initiation sequences proximal to the transcription start sites. Human tie-2 reporter constructs demonstrated approximately 10-fold greater activity in endothelial cells compared with fibroblasts. In endothelial cells the tie-2 promoter exhibited 5 and 16% of the activity of human tie-1 (830 bp) and KDR (1.1 kb) promoters, respectively. This promoter will be a useful tool for studying factors that regulate tie-2 expression and targeting the vasculature.

Promoter Regions, Genetic

Animals

Receptor Protein-Tyrosine Kinases - genetics

Receptor, TIE-2

Cattle

Endothelium, Vascular - metabolism

Base Sequence

Humans

Cells, Cultured

Cloning, Molecular

Molecular Sequence Data

Transcription, Genetic

Details

Title: Subtitle: Cloning and partial characterization of the human tie-2 receptor tyrosine kinase gene promoter
Creators: P W Hewett - CRC Academic Department of Clinical Oncology, City Hospital, Nottingham., NG5 1PB, United Kingdom. peter.hewett@nott.ac.uk
E L Daft
J C Murray
Resource Type: Journal article
Publication Details: Biochemical and biophysical research communications, Vol.252(3), pp.546-551
DOI: 10.1006/bbrc.1998.9690
PMID: 9837743
NLM abbreviation: Biochem Biophys Res Commun
ISSN: 0006-291X
eISSN: 1090-2104
Publisher: United States
Language: English
Date published: 11/27/1998
Academic Unit: Anatomy and Cell Biology; Stead Family Department of Pediatrics; Epidemiology; Pediatric Dentistry; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984025449002771

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