Journal article
Cloning and tissue-specific expression of the brain calcium channel β-subunit
FEBS letters, Vol.291(2), pp.253-258
1991
DOI: 10.1016/0014-5793(91)81296-K
PMID: 1657644
Abstract
A cDNA clone encoding a protein with high homology to the β-subunit of the rabbit skeletal muscle dihydropyridine-sensitive calcium channel was isolated from a rat brain cDNA library. This rat brain β-subunit cDNA hybridizes to a 3.4 kb message that is expressed in high levels in the cerebral hemispheres and hippocampus but is significantly reduced in cerebellum. The open reading frame encodes 597 amino acids with a predicted mass of 65 679 Da which is 82% homologous with the skeletal muscle β-subunit. The brain cDNA encodes a unique 153 amino acid C-terminus and predicts the absence of a muscle-specific 50 amino acid internal segment. It also encodes numerous consensus phosphorylation sites suggesting a role in calcium channel regulation. The corresponding human β-subunit gene was localized to chromosome 17. Hence the encoded brain β-Subunit. which has a primary structure highly similar to its isoform in skeletal muscle, may have a comparable role as an integral regulatory component of a neuronal calcium channel.
Details
- Title: Subtitle
- Cloning and tissue-specific expression of the brain calcium channel β-subunit
- Creators
- Marlon Pragnell - Program in Neuroscience, University of Iowa College of Medicine, Iowa City, IA 52242, USAJunshi Sakamoto - Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USAScott D Jay - Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA 52242, USAKevin P Campbell - Howard Hughes Medical Institute, University of Iowa College of Medicine, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.291(2), pp.253-258
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0014-5793(91)81296-K
- PMID
- 1657644
- ISSN
- 0014-5793
- eISSN
- 1873-3468
- Language
- English
- Date published
- 1991
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984020772702771
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