Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus

H L Peng; R P Novick; B Kreiswirth; J Kornblum; P Schlievert

doi:10.1128/jb.170.9.4365-4372.1988

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Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus

Journal article

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Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus

H L Peng, R P Novick, B Kreiswirth, J Kornblum and P Schlievert

Journal of bacteriology, Vol.170(9), pp.4365-4372

09/1988

DOI: 10.1128/jb.170.9.4365-4372.1988

PMCID: PMC211451

PMID: 2457579

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Abstract

We have previously identified a gene in Staphylococcus aureus, agr, whose activity is required for high-level post-exponential-phase expression of a series of secreted proteins. In this paper, we describe the cloning of this gene in Escherichia coli by using an inserted transposon (Tn551) as a cloning probe. The cloned gene, consisting of a 241-codon open reading frame containing the site of the transposon insertion, was recloned to an S. aureus vector, pSK265, and shown to be functional in S. aureus. Activity was evaluated by determinations of alpha-hemolysin, beta-hemolysin, and toxic shock syndrome toxin-1 production in early-stationary-phase cultures. The cloned gene showed considerable variation with respect to different exoproteins and different host strains compared with the chromosomal agr determinant; this variation could not be attributed to the higher copy number of the cloned gene and probably reflects inapparent subtleties of the regulatory system.

Details

Title: Subtitle: Cloning, characterization, and sequencing of an accessory gene regulator (agr) in Staphylococcus aureus
Creators: H L Peng - Public Health Research Institute, New York, New York 10016
R P Novick - Public Health Research Institute, New York, New York 10016
B Kreiswirth - Public Health Research Institute, New York, New York 10016
J Kornblum - Public Health Research Institute, New York, New York 10016
P Schlievert - Public Health Research Institute, New York, New York 10016
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.170(9), pp.4365-4372
DOI: 10.1128/jb.170.9.4365-4372.1988
PMID: 2457579
PMCID: PMC211451
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Language: English
Date published: 09/1988
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001113602771

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