Journal article
Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli
Infection and immunity, Vol.56(6), pp.1665-1667
06/1988
DOI: 10.1128/IAI.56.6.1665-1667.1988
PMCID: PMC259455
PMID: 3286506
Abstract
The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.
Details
- Title: Subtitle
- Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli
- Creators
- Gregory A Bohach - Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455Alan R HauserPatrick M Schlievert
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.56(6), pp.1665-1667
- Publisher
- United States
- DOI
- 10.1128/IAI.56.6.1665-1667.1988
- PMID
- 3286506
- PMCID
- PMC259455
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Grant note
- HL36611 / NHLBI NIH HHS
- Language
- English
- Date published
- 06/1988
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984002393702771
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