Clustering and immobilization of acetylcholine receptors by the 43-kD protein: a possible role for dystrophin-related protein

William D Phillips; Peter G Noakes; Steven L Roberds; Kevin P Campbell; John P Merlie

doi:10.1083/jcb.123.3.729

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Clustering and immobilization of acetylcholine receptors by the 43-kD protein: a possible role for dystrophin-related protein

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Clustering and immobilization of acetylcholine receptors by the 43-kD protein: a possible role for dystrophin-related protein

William D Phillips, Peter G Noakes, Steven L Roberds, Kevin P Campbell and John P Merlie

The Journal of cell biology, Vol.123(3), pp.729-740

11/01/1993

DOI: 10.1083/jcb.123.3.729

PMCID: PMC2200135

PMID: 8227135

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Abstract

Recombinant acetylcholine receptors (AChRs) expressed on the surface of cultured fibroblasts become organized into discrete membrane domains when the 43-kD postsynaptic protein (43k) is co-expressed in the same cells (Froehner, S.C., C. W. Luetje, P. B. Scotland, and J. Patrick, 1990. Neuron. 5:403-410; Phillips, W. D., M. C. Kopta, P. Blount, P. D. Gardner, J. H. Steinbach, and J. P. Merlie. 1991. Science (Wash. DC). 251:568-570). Here we show that AChRs present on the fibroblast cell surface prior to transfection of 43k are recruited into 43k-rich membrane domains. Aggregated AChRs show increased resistance to extraction with Triton X-100, suggesting a 43k-dependent linkage to the cytoskeleton. Myotubes of the mouse cell line C2 spontaneously display occasional AChR/43k-rich membrane domains that ranged in diameter up to 15 microns, but expressed many more when 43k was overexpressed following transfection of 43k cDNA. However, the membrane domains induced by recombinant 43k were predominantly small (< or = 2 microns). We were then interested in whether the cytoskeletal component, dystrophin related protein (DRP; Tinsley, J. M., D. J. Blake, A. Roche, U. Fairbrother, J. Riss, B. C. Byth, A. E. Knight, J. Kendrick-Jones, G. K. Suthers, D. R. Love, Y. H. Edwards, and K. E. Davis, 1992. Nature (Lond.). 360:591-593) contributed to the development of AChR clusters. Immunofluorescent anti-DRP staining was present at the earliest stages of AChR clustering at the neuromuscular synapse in mouse embryos and was also concentrated at the large AChR-rich domains on nontransfected C2 myotubes. Surprisingly, anti-DRP staining was concentrated mainly at the large, but not the small AChR clusters on C2 myotubes suggesting that DRP may be principally involved in permitting the growth of AChR clusters.

Details

Title: Subtitle: Clustering and immobilization of acetylcholine receptors by the 43-kD protein: a possible role for dystrophin-related protein
Creators: William D Phillips - Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110
Peter G Noakes - Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110
Steven L Roberds - Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110
Kevin P Campbell - Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110
John P Merlie - Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110
Resource Type: Journal article
Publication Details: The Journal of cell biology, Vol.123(3), pp.729-740
DOI: 10.1083/jcb.123.3.729
PMID: 8227135
PMCID: PMC2200135
ISSN: 0021-9525
eISSN: 1540-8140
Language: English
Date published: 11/01/1993
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984068381602771

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