Journal article
Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis
Integrative biology (Cambridge), Vol.4(9), pp.1090-1095
01/01/2012
DOI: 10.1039/c2ib00172a
PMID: 22772808
Abstract
This paper demonstrates an in vitro model to simulate the microenvironment of endometriosis. We used microfluidic channels with cover slips to pattern and release endometrial stromal cells (ESCs) and human peritoneal mesothelial cells (HPMCs) in a way that mimicked the pathophysiology of peritoneal endometriosis. This approach enabled observation in real time interactions between ESCs and HPMCs both in their normal and pathological states. HPMCs from control individuals were able to resist the invasion of ESCs from both control and endometriotic individuals. By contrast, HPMCs from endometriotic individuals were unable to resist the invasion of ESCs from both normal and endometriotic individuals. We further analyzed the dynamics between HPMCs and ESCs from endometriotic individuals. HPMCs from endometriotic individuals relaxed their adhesion to each other at the beginning of invasion of ESCs, lose their adhesion to the substrate and apoptosed when surrounded by ESCs. These data implicate that the peritoneal physiology may play an important role in endometriosis.
Details
- Title: Subtitle
- Co-cultured endometrial stromal cells and peritoneal mesothelial cells for an in vitro model of endometriosis
- Creators
- Zhenling Chen - Civil Aviation Administration of ChinaYi Dai - Peking Union Medical College HospitalZhe Dong - Peking Union Medical College HospitalMenghui Li - Peking Union Med Coll Hosp, Beijing 100730, Peoples R ChinaXuan Mu - CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, NanoScience and NanoTechnology,Beijing,China 100190Rui Zhang - Peking Union Medical College HospitalZhuo Wang - CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, NanoScience and NanoTechnology,Beijing,China 100190Wei Zhang - CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, NanoScience and NanoTechnology,Beijing,China 100190Jinghe Lang - Peking Union Med Coll Hosp, Beijing 100730, Peoples R ChinaJinhua Leng - Peking Union Medical College HospitalXingyu Jiang - CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, NanoScience and NanoTechnology,Beijing,China 100190
- Resource Type
- Journal article
- Publication Details
- Integrative biology (Cambridge), Vol.4(9), pp.1090-1095
- Publisher
- Oxford Univ Press
- DOI
- 10.1039/c2ib00172a
- PMID
- 22772808
- ISSN
- 1757-9694
- eISSN
- 1757-9708
- Number of pages
- 6
- Grant note
- KJCX2-YW-M15 / Chinese Academy of Sciences 30801233 / National Natural Science Foundation for Young Scholars of China; National Natural Science Foundation of China (NSFC); National Science Fund for Distinguished Young Scholars 2009CB930001; 2011CB933201 / Ministry of Science and Technology 21025520; 90813032; 20890023; GZ 614 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) 2122060 / Beijing Natural Science Foundation
- Language
- English
- Date published
- 01/01/2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering
- Record Identifier
- 9984276445702771
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