Journal article
Co-distribution of cysteine cathepsins and matrix metalloproteases in human dentin
Archives of oral biology, Vol.74, pp.101-107
02/2017
DOI: 10.1016/j.archoralbio.2016.11.011
PMID: 27923176
Abstract
•CT-B and CT-K co-occur on the dentin organic matrix, but they are differently distributed.•Both MMPs and CTs are responsible for dentin proteolytic activity.•The activity of MMPs is preponderant over CTs in sound dentin matrix.
It has been hypothesized that cysteine cathepsins (CTs) along with matrix metalloproteases (MMPs) may work in conjunction in the proteolysis of mature dentin matrix. The aim of this study was to verify simultaneously the distribution and presence of cathepsins B (CT-B) and K (CT-K) in partially demineralized dentin; and further to evaluate the activity of CTs and MMPs in the same tissue. The distribution of CT-B and CT-K in sound human dentin was assessed by immunohistochemistry. A double-immunolabeling technique was used to identify, at once, the occurrence of those enzymes in dentin. Activities of CTs and MMPs in dentin extracts were evaluated spectrofluorometrically. In addition, in situ gelatinolytic activity of dentin was assayed by zymography. The results revealed the distribution of CT-B and CT-K along the dentin organic matrix and also indicated co-occurrence of MMPs and CTs in that tissue. The enzyme kinetics studies showed proteolytic activity in dentin extracts for both classes of proteases. Furthermore, it was observed that, at least for sound human dentin matrices, the activity of MMPs seems to be predominant over the CTs one.
Details
- Title: Subtitle
- Co-distribution of cysteine cathepsins and matrix metalloproteases in human dentin
- Creators
- Polliana Mendes Candia Scaffa - Faculty of Dentistry at Piracicaba, University of Campinas, Piracicaba, 13414-903, BrazilLorenzo Breschi - Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, 40126, ItalyAnnalisa Mazzoni - Department of Medical Sciences, University of Trieste, Trieste, 34128, ItalyCristina de Mattos Pimenta Vidal - Faculty of Dentistry at Piracicaba, University of Campinas, Piracicaba, 13414-903, BrazilRosa Curci - IGM-CNR, Unit of Bologna c/o IOR, Bologna, 40136, ItalyFabianni Apolonio - Department of Pharmacy, Dentistry and Nursing, Federal University of Ceara, Fortaleza, 60020-181, BrazilPietro Gobbi - Department of SteVA, University “Carlo Bo”, Urbino, 61029, ItalyDavid Pashley - Emeritus Professor of Oral Biology, Dental College of Georgia, Georgia Health Sciences University, Augusta, Georgia, 30912, USALeo Tjäderhane - Institute of Dentistry, University of Oulu, and Medical Research Center Oulu, Oulu University Hospital and University of Oulu, Oulu FI-90014, FinlandIvarne Luis dos Santos Tersariol - Department of Biochemistry, Federal University of São Paulo, São Paulo, 04021-001, BrazilFábio Dupart Nascimento - Centro Interdisciplinar de Investigação Bioquímica, University of Mogi das Cruzes, Mogi das Cruzes, 08773-520, BrazilMarcela Rocha Carrilho - Biomaterials in Dentistry Program, Anhanguera University São Paulo (UNIAN-SP), São Paulo, 05145-200, Brazil
- Resource Type
- Journal article
- Publication Details
- Archives of oral biology, Vol.74, pp.101-107
- DOI
- 10.1016/j.archoralbio.2016.11.011
- PMID
- 27923176
- NLM abbreviation
- Arch Oral Biol
- ISSN
- 0003-9969
- eISSN
- 1879-1506
- Publisher
- Elsevier Ltd
- Grant note
- DOI: 10.13039/501100001807, name: Fundação de Amparo à Pesquisa do Estado de São Paulo, award: 2016/07996-2; DOI: 10.13039/501100003593, name: Conselho Nacional de Desenvolvimento Científico e Tecnológico, award: 312768/2013-3
- Language
- English
- Date published
- 02/2017
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984065716002771
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