Journal article
Cochlear implant imaging in the mouse and guinea pig using light-sheet microscopy
Hearing research, Vol.426, pp.108639-108639
12/2022
DOI: 10.1016/j.heares.2022.108639
PMID: 36347124
Abstract
•Light-sheet microscopy was used to examine mouse and guinea pig cochleas with and without cochlear implants.•Due to absorption and refection of light from the electrode within the cochlea, imaging quality was better after removal of the electrode.•The electrode tract was clearly visible within the scala tympani in the basal turn of the cochlea and showed encapsulation of the entire length of the implant with connective tissue.•New bone growth was also observed in the connective tissue surrounding the implant.•sTSLIM may have future utility investigating the cellular composition and related 3D architecture within the implant tract in future animal cochlear implant model studies.
Postmortem examination of the cochlea with a cochlear implant in the scala tympani presents several challenges. It is technologically difficult to section a cochlea with an implant due to the presence of its wires and metal components that are adjacent to the membranous and bony tissues of the cochlea. These metal components damage traditional steel blades of a microtome in celloidin, paraffin or frozen embedded tissues. However, plastic embedded implanted cochleas have been successfully sectioned using specialized methods (Irving et al., 2013). An alternative non-destructive method is to optically section a chemically cleared cochlea using light-sheet microscopy, which we will describe in this publication. However, since this method uses a light-sheet to section the cochlea the opaque and reflective metal components of the implant results in some artifacts in the 2D optical sections. The best image quality using light-sheet fluorescent microscopy is when the implant is removed prior to imaging.
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Details
- Title: Subtitle
- Cochlear implant imaging in the mouse and guinea pig using light-sheet microscopy
- Creators
- Peter A. Santi - University of MinnesotaShane Johnson - Weill Cornell MedicineJulian Wüster - Ilmenau, GermanyMatthew Griesbach - University of MinnesotaAlexander Claussen - Department of Otolaryngology Head and Neck Surgery, University of Iowa, Iowa City, IAJonathon Kirk - Cochlear
- Resource Type
- Journal article
- Publication Details
- Hearing research, Vol.426, pp.108639-108639
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.heares.2022.108639
- PMID
- 36347124
- ISSN
- 0378-5955
- eISSN
- 1878-5891
- Language
- English
- Date published
- 12/2022
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984366379402771
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