Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradients

Tiffany N Judice; Nathan C Nelson; Chase L Beisel; Duane C Delimont; Bernd Fritzsch; Kirk W Beisel

doi:10.1016/S1385-299X(01)00138-6

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Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradients

Journal article

Peer reviewed

Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradients

Tiffany N Judice, Nathan C Nelson, Chase L Beisel, Duane C Delimont, Bernd Fritzsch and Kirk W Beisel

Brain research. Brain research protocols, Vol.9(1), pp.65-76

2002

DOI: 10.1016/S1385-299X(01)00138-6

PMID: 11852272

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Abstract

The morphology of the organ of Corti has a radial asymmetry and also changes longitudinally from base to apex. Cellular localization of transcripts within the inner ear has relied primarily on the use of sectioned tissue with in situ hybridization. However, radial and longitudinal gradients of expression are not readily recognized using sectioned tissue owing to problems in visualization of signals with varying intensities. Herein, we describe the use of whole mount in situ hybridization for identification of cochlear longitudinal and radial expression gradients in the neurosensory epithelium, hair cells. Not only can these hair cell gradients be shown in adult tissues, but also the developmental up-regulation and down-regulation of genes and their associated spatio–temporal expression patterns can be demonstrated.

Nicotinic acetylcholine receptor α9

Expression pattern

Otoferlin

Cochlea

Hair cell

Prestin

In situ hybridization

Details

Title: Subtitle: Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradients
Creators: Tiffany N Judice - Center for Hereditary Communication Disorders, Boys Town National Research Hospital, Omaha, NE 68131, USA
Nathan C Nelson - Center for Hereditary Communication Disorders, Boys Town National Research Hospital, Omaha, NE 68131, USA
Chase L Beisel - Center for Hereditary Communication Disorders, Boys Town National Research Hospital, Omaha, NE 68131, USA
Duane C Delimont - Center for Hereditary Communication Disorders, Boys Town National Research Hospital, Omaha, NE 68131, USA
Bernd Fritzsch - Department of Biomedical Sciences, Creighton University, Omaha, NE 68178, USA
Kirk W Beisel - Center for Hereditary Communication Disorders, Boys Town National Research Hospital, Omaha, NE 68131, USA
Resource Type: Journal article
Publication Details: Brain research. Brain research protocols, Vol.9(1), pp.65-76
DOI: 10.1016/S1385-299X(01)00138-6
PMID: 11852272
NLM abbreviation: Brain Res Brain Res Protoc
ISSN: 1385-299X
Publisher: Elsevier B.V
Language: English
Date published: 2002
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070839802771

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