Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: implications for epidemiological studies

Dhruva Bhattacharya; Rashidul Haque; Upinder Singh

doi:10.1128/JCM.43.9.4815-4819.2005

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Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: implications for epidemiological studies

Journal article

Peer reviewed

Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: implications for epidemiological studies

Dhruva Bhattacharya, Rashidul Haque and Upinder Singh

Journal of clinical microbiology, Vol.43(9), pp.4815-4819

09/01/2005

DOI: 10.1128/JCM.43.9.4815-4819.2005

PMID: 16145147

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Abstract

To evaluate genetic variability among Entamoeba histolytica strains, we sequenced 9,077 bp from each of 14 isolates. The polymorphism rates from coding and noncoding regions were significantly different (0.07% and 0.37%, respectively), indicating that these regions are subject to different selection pressures. Additionally, single nucleotide polymorphisms (SNPs) potentially associated with specific clinical outcomes were identified.

Animals

Entamoeba histolytica - classification

Entamoeba histolytica - genetics

Entamoebiasis - epidemiology

Entamoebiasis - parasitology

Epidemiologic Studies

Female

Genetic Variation

Genome, Protozoan

Genotype

Humans

Male

Molecular Sequence Data

Open Reading Frames - genetics

Polymorphism, Genetic

Polymorphism, Single Nucleotide - genetics

RNA, Untranslated - genetics

Sequence Analysis, DNA

Details

Title: Subtitle: Coding and noncoding genomic regions of Entamoeba histolytica have significantly different rates of sequence polymorphisms: implications for epidemiological studies
Creators: Dhruva Bhattacharya - Department of Microbiology and Immunology, Division of Infectious Diseases, Stanford, CA 94305, USA
Rashidul Haque - International Centre for Diarrhoeal Disease Research
Upinder Singh - Stanford University
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.43(9), pp.4815-4819
DOI: 10.1128/JCM.43.9.4815-4819.2005
PMID: 16145147
ISSN: 0095-1137
eISSN: 1098-660X
Grant note: AI-053724 / NIAID NIH HHS R01 AI043596 / NIAID NIH HHS R01 AI053724 / NIAID NIH HHS AI-063470 / NIAID NIH HHS AI-43596 / NIAID NIH HHS R21 AI063470 / NIAID NIH HHS
Language: English
Date published: 09/01/2005
Academic Unit: Internal Medicine
Record Identifier: 9985244554302771

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