Journal article
Collagen-induced arthritis is exacerbated in IL-10-deficient mice
Arthritis research & therapy, Vol.5(1), pp.R18-R24
2003
DOI: 10.1186/ar601
PMCID: PMC154422
PMID: 12716449
Abstract
IL-10 is a potent immunoregulatory cytokine attenuating a wide range of immune effector and inflammatory responses. In the present study, we assess whether endogenous levels of IL-10 function to regulate the incidence and severity of collagen-induced arthritis. DBA/1 wildtype (WT), heterozygous (IL-10
+/-
) and homozygous (IL-10
-/-
) IL-10-deficient mice were immunized with type II collagen. Development of arthritis was monitored over time, and collagen-specific cytokine production and anticollagen antibodies were assessed. Arthritis developed progressively in mice immunized with collagen, and 100% of the WT, IL-10
+/-
, and IL-10
-/-
mice were arthritic at 35 days. However, the severity of arthritis in the IL-10
-/-
mice was significantly greater than that in WT or IL-1
+/-
animals. Disease severity was associated with reduced IFN-γ levels and a dramatic increase in CD11b-positive macrophages. Paradoxically, both the IgG
1
and IgG
2a
anticollagen antibody responses were also significantly reduced. These data demonstrate that IL-10 is capable of controlling disease severity through a mechanism that involves IFN-γ. Since IL-10 levels are elevated in rheumatoid arthritis synovial fluid, these findings may have relevance to rheumatoid arthritis.
Details
- Title: Subtitle
- Collagen-induced arthritis is exacerbated in IL-10-deficient mice
- Creators
- Alison Finnegan - Department of Medicine, Section of Rheumatology, Rush Presbyterian–St Luke's Medical Center, Chicago, Illinois, USACharles D Kaplan - Department of Immunology and Microbiology, Rush Presbyterian–St Luke's Medical Center, Chicago, Illinois, USAYanxia Cao - Department of Medicine, Section of Rheumatology, Rush Presbyterian–St Luke's Medical Center, Chicago, Illinois, USAHermann Eibel - Klinische Forschergruppe fur Rheumatologie, Freiburg, GermanyTibor T Glant - Department of Immunology and Microbiology, Rush Presbyterian–St Luke's Medical Center, Chicago, Illinois, USAJian Zhang - Department of Immunology and Microbiology, Rush Presbyterian–St Luke's Medical Center, Chicago, Illinois, USA
- Resource Type
- Journal article
- Publication Details
- Arthritis research & therapy, Vol.5(1), pp.R18-R24
- Publisher
- BioMed Central; London
- DOI
- 10.1186/ar601
- PMID
- 12716449
- PMCID
- PMC154422
- ISSN
- 1478-6354
- eISSN
- 1478-6362
- Language
- English
- Date published
- 2003
- Academic Unit
- Pathology
- Record Identifier
- 9984047854002771
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