Journal article
Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events
EMBO reports, Vol.5(6), pp.590-595
05/07/2004
DOI: 10.1038/sj.embor.7400150
PMCID: PMC1299070
PMID: 15133481
Abstract
Both heterotypic and homotypic fusion events are required to deliver endocytosed macromolecules to lysosomes and remodel late endocytic organelles. A
trans
-SNARE complex consisting of Q-SNAREs syntaxin 7, Vti1b and syntaxin 8 and the R-SNARE VAMP8 has been shown by others to be responsible for homotypic fusion of late endosomes. Using antibody inhibition experiments in rat liver cell-free systems, we confirmed this result, but found that the same Q-SNAREs can combine with an alternative R-SNARE, namely VAMP7, for heterotypic fusion between late endosomes and lysosomes. Co-immunoprecipitation demonstrated separate syntaxin 7 complexes with either VAMP7 or VAMP8 in solubilized rat liver membranes. Additionally, overexpression of the N-terminal domain of VAMP7, in cultured fibroblastic cells, inhibited the mixing of a preloaded lysosomal content marker with a marker delivered to late endosomes. These data show that combinatorial interactions of SNAREs determine whether late endosomes undergo homotypic or heterotypic fusion events.
Details
- Title: Subtitle
- Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events
- Creators
- Paul R Pryor - Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital Hills Road Cambridge CB2 2XY UKBarbara M Mullock - Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital Hills Road Cambridge CB2 2XY UKNicholas A Bright - Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital Hills Road Cambridge CB2 2XY UKMargaret R Lindsay - Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital Hills Road Cambridge CB2 2XY UKSally R Gray - Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital Hills Road Cambridge CB2 2XY UKSimon C W Richardson - University of IowaAbigail Stewart - Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital Hills Road Cambridge CB2 2XY UKDavid E James - Garvan Institute of Medical ResearchRobert C Piper - University of IowaJ Paul Luzio - Cambridge Institute for Medical Research and Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital Hills Road Cambridge CB2 2XY UK
- Resource Type
- Journal article
- Publication Details
- EMBO reports, Vol.5(6), pp.590-595
- DOI
- 10.1038/sj.embor.7400150
- PMID
- 15133481
- PMCID
- PMC1299070
- ISSN
- 1469-221X
- eISSN
- 1469-3178
- Language
- English
- Date published
- 05/07/2004
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297614202771
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