Journal article
Combining Volumetric Capnography And Barometric Plethysmography To Measure The Lung Structure-function Relationship
Journal of Visualized Experiments, Vol.143, e58238
2019
DOI: 10.3791/58238
PMID: 30688293
Abstract
Tools to measure lung and airways volume are critical for pulmonary researchers interested in evaluating the impact of disease or novel therapies on the lung. Barometric plethysmography is a classic technique to evaluate the lung volume with a long history of clinical use. Volumetric capnography utilizes the profile of exhaled carbon dioxide to determine the volume of the conducting airways, or dead space, and provides an index of airways homogeneity. These techniques may be used independently, or in combination to evaluate the dependence of airways volume and homogeneity on lung volume. This paper provides detailed technical instructions to replicate these techniques and our representative data demonstrates that the airways volume and homogeneity are highly correlated to lung volume. We also provide a macro for the analysis of capnographic data, which can be modified or adapted to fit different experimental designs. The advantage of these measures is that their advantages and limitations are supported by decades of experimental data, and they can be made repeatedly in the same subject without expensive imaging equipment or technically advanced analysis algorithms. These methods may be particularly useful for investigators interested in perturbations that change both the functional residual capacity of the lung and airways volume.
Details
- Title: Subtitle
- Combining Volumetric Capnography And Barometric Plethysmography To Measure The Lung Structure-function Relationship
- Creators
- McKayla Seymour - Department of Health and Human Physiology, Department of Internal Medicine, University of IowaElizabeth Pritchard - Department of Health and Human Physiology, Department of Internal Medicine, University of IowaHassan Sajjad - Pulmonary, Critical Care and Occupational Medicine Division, University of IowaErik P Tomasson - Pulmonary, Critical Care and Occupational Medicine Division, University of IowaCole M Blodgett - Department of Health and Human Physiology, Department of Internal Medicine, University of IowaHarold Winnike - Institute for Clinical and Translational Science and Stead Family Department of Pediatrics, University of IowaOana V Paun - Hematology, Oncology and Bone Marrow Transplant Division, University of IowaMichael Eberlein - Pulmonary, Critical Care and Occupational Medicine Division, University of IowaMelissa L Bates - Institute for Clinical and Translational Science and Stead Family Department of Pediatrics, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of Visualized Experiments, Vol.143, e58238
- Publisher
- MyJove Corporation
- DOI
- 10.3791/58238
- PMID
- 30688293
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Language
- English
- Date published
- 2019
- Academic Unit
- Clinical Research Unit; Stead Family Department of Pediatrics; Health and Human Physiology; Internal Medicine
- Record Identifier
- 9984002340102771
Metrics
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