Combining chondroitinase ABC and growth factors promotes the integration of murine retinal progenitor cells transplanted into Rho(-/-) mice

Jian Ma; Mostafa Kabiel; Budd A Tucker; Jian Ge; Michael J Young

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Combining chondroitinase ABC and growth factors promotes the integration of murine retinal progenitor cells transplanted into Rho(-/-) mice

Journal article

Peer reviewed

Combining chondroitinase ABC and growth factors promotes the integration of murine retinal progenitor cells transplanted into Rho(-/-) mice

Jian Ma, Mostafa Kabiel, Budd A Tucker, Jian Ge and Michael J Young

Molecular vision, Vol.17(193), pp.1759-1770

2011

PMCID: PMC3133841

PMID: 21750603

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Abstract

The aim of this study is to investigate the synergistic effect of chondroitinase ABC and growth factors in the integration of murine retinal progenitor cells (mRPCs) transplanted into Rho(-/-) mice. mRPCs from P1 green fluorescent protein-transgenic mice were isolated and expanded for transplantation. All mRPCs of 20 passages or less were transplanted into the subretinal space of B6 mice together with chondroitinase ABC, and into Rho(-/-) mice combined with chondroitinase ABC, N-[N-(3, 5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT), and insulin growth factor (IGF)-1. Cell counts were used to examine the migration and survival rate of mRPCs in B6 mice. Immunohistochemistry was used to evaluate the differentiation and integration of mRPCs in B6 and Rho(-/-) mice. Our results show that substantial numbers of mRPCs migrated and survived in the retina when transplanted with chondroitinase ABC into B6 and Rho(-/-) mice. Chondroitinase ABC disrupted the glial scar around the mRPCs in the subretinal space. Only a few mRPCs expressed recoverin in B6 mice. More mRPCs expressed rhodopsin, recoverin, and synaptophysin after transplantation into Rho(-/-) mice when combined with chondroitinase ABC and growth factors. The synergistic effect of chondroitinase ABC and growth factors facilitates the anatomic integration of mRPCs transplanted into Rho(-/-) mice.

Retina - drug effects

Retina - transplantation

Cell Count

Chondroitin ABC Lyase - administration & dosage

Green Fluorescent Proteins - genetics

Stem Cells - cytology

Stem Cell Transplantation

Retina - cytology

Insulin-Like Growth Factor I - administration & dosage

Genes, Reporter

Cell Survival - drug effects

Green Fluorescent Proteins - analysis

Cell Separation

Mice, Inbred C57BL

Mice, Knockout

Drug Synergism

Cell Movement - drug effects

Dipeptides - administration & dosage

Animals

Rhodopsin - genetics

Cell Differentiation - drug effects

Rhodopsin - deficiency

Stem Cells - physiology

Mice

Graft Survival - drug effects

Drug Combinations

Details

Title: Subtitle: Combining chondroitinase ABC and growth factors promotes the integration of murine retinal progenitor cells transplanted into Rho(-/-) mice
Creators: Jian Ma - Sun Yat-sen University
Mostafa Kabiel
Budd A Tucker
Jian Ge
Michael J Young
Resource Type: Journal article
Publication Details: Molecular vision, Vol.17(193), pp.1759-1770
Publisher: United States
PMID: 21750603
PMCID: PMC3133841
ISSN: 1090-0535
eISSN: 1090-0535
Language: English
Date published: 2011
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9983980286202771

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36 Times Cited - Web of Science