Journal article
Common Expression of Varicella-Zoster Viral Glycoprotein Antigens in Vitro and in Chickenpox and Zoster Vesicles
The Journal of infectious diseases, Vol.148(4), pp.630-638
10/1983
DOI: 10.1093/infdis/148.4.630
PMID: 6313814
Abstract
Human cells infected with varicella-zoster virus (VZV) produce at least three major virus-specific, immunogenic glycoproteins: gp118, gp98, and gp62. Since glycoproteins gp98 and gp62 were found to be prominent constituents of the infected cell membrane, a murine monoclonal antibody (clone 3B3) that reacted avidly with this glycoprotein complex was selected as a probe for detection of VZV replication in laboratory and clinical settings. Cultured cells of human, simian, and caviid origin, when infected with wild-type isolates as well as laboratory and vaccine strains of VZV all expressed these viral glycoproteins. The monoclonal antibody immunostained the basal and malpighian epithelial layers of a zoster vesicle biopsy specimen and also reacted with all specimens of vesicular cells obtained from epidemiologically unrelated patients with chickenpox and zoster. Thus, these studies demonstrate that the VZV-specific glycoprotein complex gp98/gp62 is highly conserved, abundantly expressed, and easily detected with a monoclonal antibody probe.
Details
- Title: Subtitle
- Common Expression of Varicella-Zoster Viral Glycoprotein Antigens in Vitro and in Chickenpox and Zoster Vesicles
- Creators
- Kristen A Weigle - Departments of Pediatrics and Microbiology, University of Texas Health Science Center, San Antonio, TexasCharles Grose - Departments of Pediatrics and Microbiology, University of Texas Health Science Center, San Antonio, Texas
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.148(4), pp.630-638
- Publisher
- The University of Chicago Press
- DOI
- 10.1093/infdis/148.4.630
- PMID
- 6313814
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 10/1983
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093490202771
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