Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil

D.G. Teixeira; G.R.G. Monteiro; D.R.A. Martins; M.Z. Fernandes; V. Macedo-Silva; M. Ansaldi; P.R.P. Nascimento; M.A. Kurtz; J.A. Streit; M.F.F.M. Ximenes; R.D. Pearson; A. Miles; J.M. Blackwell; M.E. Wilson; A. Kitchen; J.E. Donelson; J.P.M.S. Lima; S.M.B. Jeronimo

doi:10.1016/j.ijpara.2017.04.004

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Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil

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Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil

D.G. Teixeira, G.R.G. Monteiro, D.R.A. Martins, M.Z. Fernandes, V. Macedo-Silva, M. Ansaldi, P.R.P. Nascimento, M.A. Kurtz, J.A. Streit, M.F.F.M. Ximenes, …

International journal for parasitology, Vol.47(10-11), pp.655-665

09/01/2017

DOI: 10.1016/j.ijpara.2017.04.004

PMID: 28606698

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https://admin.research-repository.uwa.edu.au/en/publications/c063ab6f-95b2-43e5-8195-51739c1b2f73View

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Abstract

[Display omitted] •Leishmania infantum genomes were compared according their temporal isolation.•Asymptomatic L. infantum Isolates are less diverse than the symptomatic ones.•Chromosome somy varies dramatically amongst the different genomes.•At least two populations of L. infantum are circulating in northeastern Brazil. The genomic sequences of 20 Leishmania infantum isolates collected in northeastern Brazil were compared with each other and with the available genomic sequences of 29 L. infantum/donovani isolates from Nepal and Turkey. The Brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. Two isolates were from the blood and bone marrow of the same visceral leishmaniasis patient. All 20 genomic sequences display 99.95% identity with each other and slightly less identity with a reference L. infantum genome from a Spanish isolate. Despite the high identity, analysis of individual differences among the 32 million base pair genomes showed sufficient variation to allow the isolates to be clustered based on the primary sequence. A major source of variation detected was in chromosome somy, with only four of the 36 chromosomes being predominantly disomic in all 49 isolates examined. In contrast, chromosome 31 was predominantly tetrasomic/pentasomic, consistent with its regions of synteny on two different disomic chromosomes of Trypanosoma brucei. In the Brazilian isolates, evidence for recombination was detected in 27 of the 36 chromosomes. Clustering analyses suggested two populations, in which two of the five older isolates from the 1990s clustered with a majority of recent isolates. Overall the analyses do not suggest individual sequence variants account for differences in clinical outcome or adaptation to different hosts. For the first known time, DNA of isolates from asymptomatic subjects were sequenced. Of interest, these displayed lower diversity than isolates from symptomatic subjects, an observation that deserves further investigation with additional isolates from asymptomatic subjects.

Brazil

Evolution

Copy number variation

Intracellular parasite

Leishmania infantum

Parasite population structure

Details

Title: Subtitle: Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil
Creators: D.G. Teixeira - Instituto Federal do Rio Grande do Norte
G.R.G. Monteiro - Institute of Tropical Medicine of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil
D.R.A. Martins - Universidade Federal do Rio Grande do Norte
M.Z. Fernandes - Universidade Federal do Rio Grande do Norte
V. Macedo-Silva - Department of Biochemistry, Bioscience Center, Federal University of Rio Grande do Norte, Natal, RN, Rio Grande do Norte, Brazil
M. Ansaldi - Universidade Federal do Rio Grande do Norte
P.R.P. Nascimento - Universidade Federal do Rio Grande do Norte
M.A. Kurtz - Veterans’ Affairs Medical Center, Iowa City, IA, USA
J.A. Streit - University of Iowa, Infectious Diseases
M.F.F.M. Ximenes - Universidade Federal do Rio Grande do Norte
R.D. Pearson - University of Virginia
A. Miles - Centre for Human Genetics
J.M. Blackwell - The Kids Research Institute Australia
M.E. Wilson - University of Iowa, Infectious Diseases
A. Kitchen - University of Iowa, Anthropology
J.E. Donelson - University of Iowa, Biochemistry and Molecular Biology
J.P.M.S. Lima - Instituto Federal do Rio Grande do Norte
S.M.B. Jeronimo - Department of Biochemistry, Bioscience Center, Federal University of Rio Grande do Norte, Natal, RN, Rio Grande do Norte, Brazil
Resource Type: Journal article
Publication Details: International journal for parasitology, Vol.47(10-11), pp.655-665
DOI: 10.1016/j.ijpara.2017.04.004
PMID: 28606698
NLM abbreviation: Int J Parasitol
ISSN: 0020-7519
eISSN: 1879-0135
Publisher: Elsevier Ltd
Number of pages: 11
Language: English
Date published: 09/01/2017
Academic Unit: Anthropology; Microbiology and Immunology; International Programs; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984001203102771

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