Journal article
Comparative analysis of human tear fluid and aqueous humor proteomes
The ocular surface, Vol.33, pp.16-22
07/01/2024
DOI: 10.1016/j.jtos.2024.03.011
PMCID: PMC11179983
PMID: 38561100
Abstract
Technological advancements allowing for the analysis of low-volume samples have led to the investigation of human tear fluid and aqueous humor (AH) as potential biomarker sources. However, acquiring AH samples poses significant challenges, making human tear fluid a more accessible alternative. This study aims to compare the protein compositions of these two biofluids to evaluate their suitability for biomarker discovery.
Paired tear and AH samples were collected from 20 patients undergoing cataract surgery. Tear samples were collected using Schirmer strips prior to surgery, and AH samples were collected from the anterior chamber immediately after corneal incision. Proteins were extracted and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
A total of 481 proteins were identified in greater than 50% of the tear samples, and 191 proteins were detected in greater than 50% of the AH samples. Of these proteins, 82 were found to be common between the two biofluids, with ALB, LTF, TF, LCN1, and IGKC being the most abundant.
Although tear fluid and the AH are functionally independent and physically separated, many of the proteins detected in AH were also detected in tears. This direct comparison of the proteomic content of tear fluid and AH may aid in further investigation of tear fluid as a source of readily accessible biomarkers for various human diseases.
Details
- Title: Subtitle
- Comparative analysis of human tear fluid and aqueous humor proteomes
- Creators
- August Beisel - Augusta UniversityGarrett Jones - Augusta UniversityJoshua Glass - Augusta UniversityTae Jin Lee - Augusta UniversityMarc Töteberg-Harms - Augusta UniversityAmy Estes - Augusta UniversityLane Ulrich - Augusta UniversityKathryn Bollinger - Augusta UniversityShruti Sharma - Augusta UniversityAshok Sharma - Augusta University
- Resource Type
- Journal article
- Publication Details
- The ocular surface, Vol.33, pp.16-22
- DOI
- 10.1016/j.jtos.2024.03.011
- PMID
- 38561100
- PMCID
- PMC11179983
- NLM abbreviation
- Ocul Surf
- ISSN
- 1542-0124
- eISSN
- 1937-5913
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 07/01/2024
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984696717102771
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