Journal article
Comparative analysis of three murine G-protein coupled receptors activated by sphingosine-1-phosphate
Gene, Vol.227(1), pp.89-99
1999
DOI: 10.1016/S0378-1119(98)00589-7
PMID: 9931453
Abstract
The cloning and analysis of the first identified lysophosphatidic acid (LPA) receptor gene,
lp
A1 (also referred to as
vzg-1 or
edg-2), led us to identify homologous murine genes that might also encode receptors for related lysophospholipid ligands. Three murine genomic clones (designated
lp
B1,
lp
B2, and
lp
B3) were isolated, corresponding to human/rat
Edg-1, rat H218/AGR16, and human edg-3, respectively. Based on the amino acid similarities of their predicted proteins (44–52% identical), the three
lp
B genes could be grouped into a separate G-protein coupled receptor subfamily, distinct from that containing the LPA receptor genes
lp
A1 and
lp
A2. Unlike
lp
A1 and
lp
A2, which contain multiple coding exons, all
lp
B members contained a single coding exon. Heterologous expression of individual
lp
B members in a hepatoma cell line (RH7777), followed by
35S-GTPγS
incorporation assays demonstrated that each of the three LP
B receptors conferred sphingosine-1-phosphate-dependent, but not lysophosphatidic acid-dependent, G-protein activation. Northern blot and in situ hybridization analyses revealed overlapping as well as distinct expression patterns in both embryonic and adult tissues. This comparative characterization of multiple sphingosine-1-phosphate receptor genes and their spatiotemporal expression patterns will aid in understanding the biological roles of this enlarging lysophospholipid receptor family.
Details
- Title: Subtitle
- Comparative analysis of three murine G-protein coupled receptors activated by sphingosine-1-phosphate
- Creators
- Guangfa Zhang - Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0636, USAJames J.A Contos - Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0636, USAJoshua A Weiner - Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0636, USANobuyuki Fukushima - Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0636, USAJerold Chun - Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla, CA 92093-0636, USA
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.227(1), pp.89-99
- DOI
- 10.1016/S0378-1119(98)00589-7
- PMID
- 9931453
- NLM abbreviation
- Gene
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1999
- Academic Unit
- Liberal Arts and Science Admin; Psychiatry; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9983997436402771
Metrics
71 Record Views