Comparative digital cartilage histology for human and common osteoarthritis models

Douglas R Pedersen; Jessica E Goetz; Gail L Kurriger; James A Martin

doi:10.2147/ORR.S38400

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Comparative digital cartilage histology for human and common osteoarthritis models

Journal article

Open access

Peer reviewed

Comparative digital cartilage histology for human and common osteoarthritis models

Douglas R Pedersen, Jessica E Goetz, Gail L Kurriger and James A Martin

Orthopedic research and reviews, Vol.2013(5), pp.13-20

02/12/2013

DOI: 10.2147/ORR.S38400

PMCID: PMC3899351

PMID: 24465137

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Abstract

This study addresses the species-specific and site-specific details of weight-bearing articular cartilage zone depths and chondrocyte distributions among humans and common osteoarthritis (OA) animal models using contemporary digital imaging tools. Histological analysis is the gold-standard research tool for evaluating cartilage health, OA severity, and treatment efficacy. Historically, evaluations were made by expert analysts. However, state-of-the-art tools have been developed that allow for digitization of entire histological sections for computer-aided analysis. Large volumes of common digital cartilage metrics directly complement elucidation of trends in OA inducement and concomitant potential treatments. Sixteen fresh human knees, 26 adult New Zealand rabbit stifles, and 104 bovine lateral plateaus were measured for four cartilage zones and the cell densities within each zone. Each knee was divided into four weight-bearing sites: the medial and lateral plateaus and femoral condyles. One-way analysis of variance followed by pairwise multiple comparisons (Holm-Sidak method at a significance of 0.05) clearly confirmed the variability between cartilage depths at each site, between sites in the same species, and between weight-bearing articular cartilage definitions in different species. The present study clearly demonstrates multisite, multispecies differences in normal weight-bearing articular cartilage, which can be objectively quantified by a common digital histology imaging technique. The clear site-specific differences in normal cartilage must be taken into consideration when characterizing the pathoetiology of OA models. Together, these provide a path to consistently analyze the volume and variety of histologic slides necessarily generated by studies of OA progression and potential treatments in different species.

cell density

knee

bovine

imaging

osteoarthritis

rabbit

Details

Title: Subtitle: Comparative digital cartilage histology for human and common osteoarthritis models
Creators: Douglas R Pedersen - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, USA
Jessica E Goetz - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, USA
Gail L Kurriger - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, USA
James A Martin - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Orthopedic research and reviews, Vol.2013(5), pp.13-20
DOI: 10.2147/ORR.S38400
PMID: 24465137
PMCID: PMC3899351
NLM abbreviation: Orthop Res Rev
ISSN: 1179-1462
eISSN: 1179-1462
Publisher: New Zealand
Grant note: P50 AR055533 / NIAMS NIH HHS
Language: English
Date published: 02/12/2013
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation
Record Identifier: 9984040256202771

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