Journal article
Comparative evaluation of an automated ribotyping system versus pulsed-field gel electrophoresis for epidemiological typing of clinical isolates of Escherichia coli and Pseudomonas aeruginosa from patients with recurrent gram-negative bacteremia
Diagnostic microbiology and infectious disease, Vol.25(1), pp.1-8
1996
DOI: 10.1016/0732-8893(96)00082-X
PMID: 8831038
Abstract
Ribotyping and macrorestriction analysis of chromosomal DNA using pulsed-field gel electrophoresis (PFGE) are among the more useful molecular epidemiologic typing methods. Because these techniques are labor intensive, automation of one or more steps may allow clinical laboratories to apply molecular typing methods. We compared the recently developed automated ribotyping system, the RiboPrinter™ Microbial Characterization System (DuPont), with PFGE as a means of typing clinical isolates of
E. coli and
P. aeruginosa. A total of 22
E. coli and 24
P. aeruginosa were typed by both PFGE and the RiboPrinter™. When compared with PFGE typing of
E. coli and
P. aeruginosa, the RiboPrinter™ was less sensitive in identifying different strains, particularly among the isolates of
P. aeruginosa. The RiboPrinter™ was completely automated and allowed up to 32 isolates to be typed within an 8-hour period. The pattern of results obtained in this study suggests that a hierarchical approach to molecular typing using the RiboPrinter™ Microbial Characterization System plus PFGE might be feasible. The RiboPrinter™ Microbial Characterization System promises to be a very useful addition to the expanding molecular typing armamentarium.
Details
- Title: Subtitle
- Comparative evaluation of an automated ribotyping system versus pulsed-field gel electrophoresis for epidemiological typing of clinical isolates of Escherichia coli and Pseudomonas aeruginosa from patients with recurrent gram-negative bacteremia
- Creators
- M.A Pfaller - From the Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242 USAC Wendt - From the Department of International Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242 USAR.J Hollis - From the Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242 USAR.P Wenzel - From the Department of International Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242 USAS.J Fritschel - E.I. duPont de Nemours and Company Wilmington, Delaware 19880-0402 USAJ.J Neubauer - E.I. duPont de Nemours and Company Wilmington, Delaware 19880-0402 USAL.A Herwaldt - From the Department of International Medicine, University of Iowa College of Medicine, Iowa City, Iowa 52242 USA
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.25(1), pp.1-8
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0732-8893(96)00082-X
- PMID
- 8831038
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 1996
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9984094585802771
Metrics
8 Record Views