Comparative gene-expression profiling of CD133(+) and CD133(-) D10 melanoma cells

Raheleh Roudi; Marzieh Ebrahimi; Mehrdad Nasrollahzadeh Sabet; Ali Najafi; Mohamad Reza Nourani; Motahareh Rajabi Fomeshi; Ali Samadikuchaksaraei; Ahmad Shariftabrizi; Zahra Madjd

doi:10.2217/fon.15.174

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Comparative gene-expression profiling of CD133(+) and CD133(-) D10 melanoma cells

Journal article

Peer reviewed

Comparative gene-expression profiling of CD133(+) and CD133(-) D10 melanoma cells

Raheleh Roudi, Marzieh Ebrahimi, Mehrdad Nasrollahzadeh Sabet, Ali Najafi, Mohamad Reza Nourani, Motahareh Rajabi Fomeshi, Ali Samadikuchaksaraei, Ahmad Shariftabrizi and Zahra Madjd

Future oncology (London, England), Vol.11(17), pp.2383-2393

2015

DOI: 10.2217/fon.15.174

PMID: 26285774

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Abstract

The present study aimed to compare the gene-expression profiling of CD133(+) and CD133(-) D10 cells. Cancer stem cell-like properties and gene-expression profiling of CD133(+) D10 cells versus CD133(-) cells were evaluated. The CD133(+) D10 cells showed significantly higher clonogenic and spheroid forming potential, also higher expression of stemness genes NANOG and OCT4A compared with the CD133(-) cells. Gene-expression profiling of CD133(+) versus CD133(-) D10 cells revealed that 130 genes including ABC transporter superfamily (ABCC1, ABCG2 and ABCC6) were upregulated, while 61 genes including apoptosis modifying genes (CASP8 and TNFRSF4) were downregulated. We conclude that many genes involved in drug resistance and tumor aggressiveness are upregulated in CD133(+) D10 cells and targeting them might be an efficient strategy for treatment of melanoma.

AC133 Antigen

Antigens, CD - genetics

Antigens, CD - metabolism

Biomarkers, Tumor

Cell Line, Tumor

Computational Biology - methods

Gene Expression Profiling

Gene Expression Regulation, Neoplastic

Glycoproteins - genetics

Glycoproteins - metabolism

Humans

Immunophenotyping

Melanoma - genetics

Melanoma - metabolism

Peptides - genetics

Peptides - metabolism

Protein Interaction Mapping

Protein Interaction Maps

Transcriptome

Details

Title: Subtitle: Comparative gene-expression profiling of CD133(+) and CD133(-) D10 melanoma cells
Creators: Raheleh Roudi - Iran University of Medical Sciences
Marzieh Ebrahimi - Royan Institute
Mehrdad Nasrollahzadeh Sabet - Iran University of Medical Sciences
Ali Najafi - Baqiyatallah University of Medical Sciences
Mohamad Reza Nourani - Genomics Division, Systems Biology Institute, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Motahareh Rajabi Fomeshi - Royan Institute
Ali Samadikuchaksaraei - Iran University of Medical Sciences
Ahmad Shariftabrizi - University at Buffalo, State University of New York
Zahra Madjd - Iran University of Medical Sciences
Resource Type: Journal article
Publication Details: Future oncology (London, England), Vol.11(17), pp.2383-2393
DOI: 10.2217/fon.15.174
PMID: 26285774
ISSN: 1479-6694
eISSN: 1744-8301
Language: English
Date published: 2015
Academic Unit: Radiology
Record Identifier: 9984318792702771

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