Comparative genetics of Enterococcus faecalis intestinal tissue isolates before and after surgery in a rat model of colon anastomosis

Scott Christley; Benjamin Shogan; Zoe Levine; Hyun Koo; Kristina Guyton; Sarah Owens; Jack Gilbert; Olga Zaborina; John C. Alverdy

doi:10.1371/journal.pone.0232165

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Comparative genetics of Enterococcus faecalis intestinal tissue isolates before and after surgery in a rat model of colon anastomosis

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Comparative genetics of Enterococcus faecalis intestinal tissue isolates before and after surgery in a rat model of colon anastomosis

Scott Christley, Benjamin Shogan, Zoe Levine, Hyun Koo, Kristina Guyton, Sarah Owens, Jack Gilbert, Olga Zaborina and John C. Alverdy

PloS one, Vol.15(4), pp.e0232165-e0232165

04/28/2020

DOI: 10.1371/journal.pone.0232165

PMCID: PMC7188289

PMID: 32343730

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Abstract

We have recently demonstrated that collagenolytic Enterococcus faecalis plays a key and causative role in the pathogenesis of anastomotic leak, an uncommon but potentially lethal complication characterized by disruption of the intestinal wound following segmental removal of the colon (resection) and its reconnection (anastomosis). Here we hypothesized that comparative genetic analysis of E. faecalis isolates present at the anastomotic wound site before and after surgery would shed insight into the mechanisms by which collagenolytic strains are selected for and predominate at sites of anastomotic disruption. Whole genome optical mapping of four pairs of isolates from rat colonic tissue obtained following surgical resection (herein named "pre-op" isolates) and then 6 days later from the anastomotic site (herein named "post-op" isolates) demonstrated that the isolates with higher collagenolytic activity formed a distinct cluster. In order to perform analysis at a deeper level, a single pair of E. faecalis isolates (16A pre-op and 16A post-op) was selected for whole genome sequencing and assembled using a hybrid assembly algorithm. Comparative genomics demonstrated absence of multiple gene clusters, notably a pathogenicity island in the post-op isolate. No differences were found in the fsr-gelE-sprE genes (EF1817-1822) responsible for regulation and production of collagenolytic activity. Analysis of unique genes among the 16A pre-op and post-op isolates revealed the predominance of transporter systems-related genes in the pre-op isolate and phage-related and hydrolytic enzyme-encoding genes in the post-op isolate. Despite genetic differences observed between pre-op and post-op isolates, the precise genetic determinants responsible for their differential expression of collagenolytic activity remains unknown.

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Title: Subtitle: Comparative genetics of Enterococcus faecalis intestinal tissue isolates before and after surgery in a rat model of colon anastomosis
Creators: Scott Christley - University of Chicago
Benjamin Shogan - University of Chicago
Zoe Levine - University of Chicago
Hyun Koo - University of Chicago
Kristina Guyton - University of Chicago
Sarah Owens - Argonne National Laboratory
Jack Gilbert - Argonne National Laboratory
Olga Zaborina - University of Chicago
John C. Alverdy - University of Chicago
Resource Type: Journal article
Publication Details: PloS one, Vol.15(4), pp.e0232165-e0232165
DOI: 10.1371/journal.pone.0232165
PMID: 32343730
PMCID: PMC7188289
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library Science
Number of pages: 13
Grant note: R01-GM062344-18 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
Language: English
Date published: 04/28/2020
Academic Unit: Surgery
Record Identifier: 9984322939002771

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