Journal article
Comparative genomic hybridization, allelic imbalance, and fluorescence in situ hybridization on chromosome 8 in prostate cancer
Genes chromosomes & cancer, Vol.11(3), pp.153-162
11/1994
DOI: 10.1002/gcc.2870110304
PMID: 7530484
Abstract
Due to problems with primary tumor cell culture, conventional cytogenetics has yielded little insightful information on chromosomal alterations in prostate cancer. The primary aim of this study was to define the ability of comparative genomic hybridization (CGH) to detect and map genetic deletions in prostate tumors. A secondary aim was to apply multiple assays to individual tumors as a means of deciphering the mechanisms of genetic alterations in prostate cancer. CGH results were compared with allelic imbalance measurements at 29 distinct loci on chromosome 8 in 18 specimens (17 malignant and 1 benign). CGH detected no changes in cases where all informative PCR/RFLP loci were retained and detected all p arm deletions consisting of at least two loci. We estimate that in this study, the smallest deletions detected by CGH were approximately 20‐30 cM. Physical mapping of subchromosomal arm deletions by CGH correlated well with allelic imbalance mapping by PCR/RFLP: The data agreed at 88% of loci on 8p and 92% of loci on 8q. Fluorescence in situ hybridization (FISH) with multiple centromere probes and DNA content flow cytometry (FCM) also was performed on selected specimens. FISH revealed two cases of chromosome 8 aneusomy. In these two cases and three others, CGH showed simultaneous p arm deletion and q arm gain, suggesting isochromosome 8q formation. Together, these data suggested that simple chromosomal aberrations were responsible for allelic losses on 8p and allelic gains on 8q in a significant number of prostate tumors. We also used CGH to examine relative DNA sequence copy number throughout the genome. Changes frequently associated with 8p loss include gains of 8q and losses of 13q, 16p, 16q, 17p, 17q, 20q, and Y. Cases with 8p loss exhibited five times the number of alterations as did cases without 8p loss. Copyright © 1994 Wiley‐Liss, Inc., A Wiley Company
Details
- Title: Subtitle
- Comparative genomic hybridization, allelic imbalance, and fluorescence in situ hybridization on chromosome 8 in prostate cancer
- Creators
- Michael L. Cher - University of California, San FranciscoDonal Macgrogan - Department of Molecular Biology, Canji Inc., San Diego, CaliforniaRobert Bookstein - Department of Molecular Biology, Canji Inc., San Diego, CaliforniaJames A. Brown - Mayo ClinicRobert B. Jenkins - University of California, San FranciscoRonald H. Jensen - Mayo Clinic
- Resource Type
- Journal article
- Publication Details
- Genes chromosomes & cancer, Vol.11(3), pp.153-162
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/gcc.2870110304
- PMID
- 7530484
- ISSN
- 1045-2257
- eISSN
- 1098-2264
- Number of pages
- 10
- Language
- English
- Date published
- 11/1994
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Urology
- Record Identifier
- 9984319975902771
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