Journal article
Comparative processing and function of human and ferret cystic fibrosis transmembrane conductance regulator
The Journal of biological chemistry, Vol.287(26), pp.21673-21685
06/22/2012
DOI: 10.1074/jbc.M111.336537
PMCID: PMC3381131
PMID: 22570484
Abstract
The most common cystic fibrosis transmembrane conductance regulator (CFTR) gene mutation is ΔF508, and this causes cystic fibrosis (CF). New CF models in the pig and ferret have been generated that develop lung, pancreatic, liver, and intestinal pathologies that reflect disease in CF patients. Species-specific biology in the processing of CFTR has demonstrated that pig and mouse ΔF508-CFTR proteins are more effectively processed to the apical membrane of airway epithelia than human ΔF508-CFTR. The processing behavior of ferret WT- and ΔF508-CFTR proteins remains unknown, and such information is important to predicting the utility of a ΔF508-CFTR ferret. To this end, we sought to compare processing, membrane stability, and function of human and ferret WT- and ΔF508-CFTR proteins in a heterologous expression system using HT1080, HEK293T, BHK21, and Cos7 cells as well as human and ferret CF polarized airway epithelia. Analysis of the protein processing and stability by metabolic pulse-chase and surface On-Cell Western blots revealed that WT-fCFTR half-life and membrane stability were increased relative to WT-hCFTR. Furthermore, in BHK21, Cos7, and CuFi cells, human and ferret ΔF508-CFTR processing was negligible, whereas low levels of processing of ΔF508-fCFTR could be seen in HT1080 and HEK293T cells. Only the WT-fCFTR, but not ΔF508-fCFTR, produced functional cAMP-inducible chloride currents in both CF human and ferret airway epithelia. Further elucidation of the mechanism responsible for elevated fCFTR protein stability may lead to new therapeutic approaches to augment CFTR function. These findings also suggest that generation of a ferret CFTR(ΔF508/ΔF508) animal model may be useful.
Details
- Title: Subtitle
- Comparative processing and function of human and ferret cystic fibrosis transmembrane conductance regulator
- Creators
- John T Fisher - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, Iowa 52242, USAXiaoming LiuZiying YanMeihui LuoYulong ZhangWeihong ZhouBen J LeeYi SongChenhong GuoYujiong WangGergely L LukacsJohn F Engelhardt
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.287(26), pp.21673-21685
- DOI
- 10.1074/jbc.M111.336537
- PMID
- 22570484
- PMCID
- PMC3381131
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- United States
- Grant note
- Canadian Institutes of Health Research R01 HL108902 / NHLBI NIH HHS P01 HL091842 / NHLBI NIH HHS DK047967 / NIDDK NIH HHS HL091842 / NHLBI NIH HHS HL108902 / NHLBI NIH HHS R37 DK047967 / NIDDK NIH HHS R01 DK047967 / NIDDK NIH HHS DK075302 / NIDDK NIH HHS R01 DK075302 / NIDDK NIH HHS P30 DK054759 / NIDDK NIH HHS DK54759 / NIDDK NIH HHS
- Language
- English
- Date published
- 06/22/2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025337902771
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