Journal article
Comparative proteomic profiling of patients with atopic dermatitis based on history of eczema herpeticum infection and Staphylococcus aureus colonization
Journal of allergy and clinical immunology, Vol.127(1), pp.186-193.e11
2011
DOI: 10.1016/j.jaci.2010.10.033
PMCID: PMC3059191
PMID: 21211653
Abstract
Atopic dermatitis (AD) is the most common inflammatory skin disorder in the general population worldwide, and the majority of patients are colonized with
Staphylococcus aureus. Eczema herpeticum is a disseminated herpes simplex virus infection that occurs in a small subset of patients.
The goal was to conduct proteomic profiling of patients with AD based on
S aureus colonization status and history of eczema herpeticum. We hoped to identify new biomarkers for improved diagnosis and prediction of eczema herpeticum and
S aureus susceptibility and to generate new hypotheses regarding disease pathogenesis.
Skin taping was performed on nonlesional skin of nonatopic control subjects and on lesional and nonlesional skin of patients with AD. Subjects were classified according to the history of eczema herpeticum and
S aureus colonization. Proteins were analyzed by using mass spectrometry; diagnostic groups were compared for statistically significant differences in protein expression.
Proteins related to the skin barrier (filaggrin-2, corneodesmosin, desmoglein-1, desmocollin-1, and transglutaminase-3) and generation of natural moisturizing factor (arginase-1, caspase-14, and gamma-glutamyl cyclotransferase) were expressed at significantly lower levels in lesional versus nonlesional sites of patients with AD with and without history of eczema herpeticum; epidermal fatty acid–binding protein was expressed at significantly higher levels in patients with methicillin-resistant
S aureus.
This noninvasive, semiquantitative profiling method has revealed novel proteins likely involved in the pathogenesis of AD. The lower expression of skin barrier proteins and enzymes involved in the generation of the natural moisturizing factor could further exacerbate barrier defects and perpetuate water loss from the skin. The greater expression of epidermal fatty acid–binding protein, especially in patients colonized with methicillin-resistant
S aureus, might perpetuate the inflammatory response through eicosanoid signaling.
Details
- Title: Subtitle
- Comparative proteomic profiling of patients with atopic dermatitis based on history of eczema herpeticum infection and Staphylococcus aureus colonization
- Creators
- Carolyn J Broccardo - Department of Immunology, National Jewish Health, Denver, ColoSpencer Mahaffey - Department of Immunology, National Jewish Health, Denver, ColoJohn Schwarz - Rho, Inc, Chapel Hill, NCLisa Wruck - Rho, Inc, Chapel Hill, NCGloria David - Rho, Inc, Chapel Hill, NCPatrick M Schlievert - Department of Microbiology, University of Minnesota Medical School, Minneapolis, MinnNichole A Reisdorph - Department of Immunology, National Jewish Health, Denver, ColoDonald Y.M Leung - Department of Pediatrics, National Jewish Health, Denver, Colo
- Resource Type
- Journal article
- Publication Details
- Journal of allergy and clinical immunology, Vol.127(1), pp.186-193.e11
- DOI
- 10.1016/j.jaci.2010.10.033
- PMID
- 21211653
- PMCID
- PMC3059191
- NLM abbreviation
- J Allergy Clin Immunol
- ISSN
- 0091-6749
- eISSN
- 1097-6825
- Publisher
- Mosby, Inc
- Grant note
- N01-AI-40029, N01 AI-40033, R01 AR41256; NCRR S10RR023703 / National Institutes of Health/National Institute of Allergy and Infectious Diseases National Institute of Allergy and Infectious Diseases
- Language
- English
- Date published
- 2011
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001161402771
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