Journal article
Comparative study reveals better far-red fluorescent protein for whole body imaging
Scientific reports, Vol.5(1), 10332
06/02/2015
DOI: 10.1038/srep10332
PMCID: PMC4603699
PMID: 26035795
Abstract
Genetically encoded far-red and near-infrared fluorescent proteins enable efficient imaging in studies of tumorigenesis, embryogenesis, and inflammation in model animals. Here we report comparative testing of available GFP-like far-red fluorescent proteins along with a modified protein, named Katushka2S, and near-infrared bacterial phytochrome-based markers. We compare fluorescence signal and signal-to-noise ratio at various excitation wavelength and emission filter combinations using transiently transfected cell implants in mice, providing a basis for rational choice of optimal marker(s) for in vivo imaging studies. We demonstrate that the signals of various far-red fluorescent proteins can be spectrally unmixed based on different signal-to-noise ratios in different channels, providing the straightforward possibility of multiplexed imaging with standard equipment. Katushka2S produced the brightest and fastest maturing fluorescence in all experimental setups. At the same time, signal-to-noise ratios for Katushka2S and near-infrared bacterial phytochrome, iRFP720 were comparable in their optimal channels. Distinct spectral and genetic characteristics suggest this pair of a far-red and a near-infrared fluorescent protein as an optimal combination for dual color, whole body imaging studies in model animals.
Details
- Title: Subtitle
- Comparative study reveals better far-red fluorescent protein for whole body imaging
- Creators
- K E LukerP Pata - Tallinn University of TechnologyIi ShemiakinaA Pereverzeva - Institute of Bioorganic ChemistryA C StacerDs ShcherboVz PletnevM Skolnaja - Tallinn University of TechnologyKa LukyanovG D LukerI Pata - Tallinn University of TechnologyDm Chudakov
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.5(1), 10332
- Publisher
- Nature Publishing Group
- DOI
- 10.1038/srep10332
- PMID
- 26035795
- PMCID
- PMC4603699
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Language
- English
- Date published
- 06/02/2015
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984696760502771
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