Journal article
Comparative utilization of n-3 polyunsaturated fatty acids by cultured human Y-79 retinoblastoma cells
Biochimica et biophysica acta, Vol.795(2), pp.277-285
1984
DOI: 10.1016/0005-2760(84)90076-6
PMID: 6089899
Abstract
The Y-79 retinoblastoma cell, a cultured human line derived from the retina, was utilized as a model for investigating the metabolism of n-3 polyunsaturated fatty acids in neural tissue. When cultures were incubated with 5 microM linolenic (18:3), eicosapentaenoic (20:5) or docosahexaenoic (22:6) acids, a low concentration probably representative of physiologic levels, the amount incorporated was 20:5 congruent to 18.3 greater than 22:6. Regardless of which fatty acid was provided, 65-75% of the total uptake accumulated in phosphatidylethanolamine and ethanolamine plasmalogen, suggesting that these phospholipids play an important role in n-3 polyunsaturated fatty acid metabolism. A small amount of 22:6 was converted to 20:5, which was recovered in phosphatidylinositol and phosphatidylserine. Therefore, one metabolic function of 22:6 may be to serve as an intracellular storage pool for the formation of 20:5 through retroconversion. When any of the n-3 polyunsaturates was available, the main fatty acid that accumulated in the cell phospholipids was 22:6. The extent to which 22:6 accumulated, however, depended on the particular n-3 polyunsaturated fatty acid that was available. This suggests that the 22:6 content of a neural cell, and any cellular function dependent on 22:6 content, may be regulated by changes in the type of n-3 polyunsaturate available to the nervous system.
Details
- Title: Subtitle
- Comparative utilization of n-3 polyunsaturated fatty acids by cultured human Y-79 retinoblastoma cells
- Creators
- Mark A Yorek - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United StatesRobin R Bohnker - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United StatesDavid T Dudley - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United StatesArthur A Spector - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Biochimica et biophysica acta, Vol.795(2), pp.277-285
- Publisher
- Elsevier
- DOI
- 10.1016/0005-2760(84)90076-6
- PMID
- 6089899
- ISSN
- 0006-3002
- eISSN
- 1878-2434
- Language
- English
- Date published
- 1984
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094723402771
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