Journal article
Comparison of Comparative Genomic Hybridization Technologies Across Microarray Platforms
Journal of biomolecular techniques, Vol.20(2), pp.135-151
04/01/2009
PMCID: PMC2685605
PMID: 19503625
Abstract
In the 2007 Association of Biomolecular Resource Facilities Microarray Research Group project, we analyzed HL-60 DNA with five platforms: Agilent, Affymetrix 500K, Affymetrix U133 Plus 2.0, Illumina, and RPCI 19K BAC arrays. Copy number variation was analyzed using circular binary segmentation (CBS) analysis of log ratio scores from four independently assessed hybridizations of each platform. Data obtained from these platforms were assessed for reproducibility and the ability to detect formerly reported copy number variations in HL-60. In HL-60, all of the tested platforms detected genomic DNA amplification of the 8q24 locus, trisomy 18, and monosomy X; and deletions at loci 5q11.2~q31, 9p21.3~p22, 10p12~p15, 14q22~q31, and 17p12~p13.3. In the HL-60 genome, at least two of the five platforms detected five novel losses and five novel gains. This report provides guidance in the selection of platforms based on this wide-ranging evaluation of available CGH platforms.
Details
- Title: Subtitle
- Comparison of Comparative Genomic Hybridization Technologies Across Microarray Platforms
- Creators
- Susan D. HesterLaura ReidNorma NowakWendell D. JonesJoel S. ParkerKevin KnudtsonWilliam WardJay TiesmanNancy D. Denslow
- Resource Type
- Journal article
- Publication Details
- Journal of biomolecular techniques, Vol.20(2), pp.135-151
- Publisher
- The Association of Biomolecular Resource Facilities
- PMID
- 19503625
- PMCID
- PMC2685605
- ISSN
- 1524-0215
- eISSN
- 1943-4731
- Language
- English
- Date published
- 04/01/2009
- Academic Unit
- Iowa Institute of Human Genetics
- Record Identifier
- 9984627238802771
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