Journal article
Comparison of Cytomegalovirus (CMV) UL97 Gene Sequences in the Blood and Vitreous of Patients with Acquired Immunodeficiency Syndrome and CMV Retinitis
The Journal of infectious diseases, Vol.185(7), pp.861-867
04/01/2002
DOI: 10.1086/339603
PMID: 11920309
Abstract
Cytomegalovirus (CMV) resistance to ganciclovir occurs via mutations in the UL97 gene. CMV DNA, from vitreous and blood specimens and from culture isolates from 87 patients with acquired immunodeficiency syndrome and CMV retinitis who received a ganciclovir implant, was sequenced to identify the relationship between the UL97 DNA sequences in the eye and peripheral blood. There was 93.5%agreement between the UL97 gene sequences frompaired vitreous specimens and blood specimens. Sequence analysis of vitreous specimens showed that 15%(13/87) of the patients had either a ganciclovir resistance-conferring mutation or a polymorphism in the CMV UL97 gene. Eleven of the 13 mutations or polymorphisms in the vitreous also were identified in blood. Although the number of mutations limits definitive interpretation, these data suggest that blood specimens may reflect the events occurring in the eyes of patients with CMV retinitis.
Details
- Title: Subtitle
- Comparison of Cytomegalovirus (CMV) UL97 Gene Sequences in the Blood and Vitreous of Patients with Acquired Immunodeficiency Syndrome and CMV Retinitis
- Creators
- Huiling Hu - Johns Hopkins University School of MedicineDouglas A Jabs - Johns Hopkins UniversityMichael S Forman - Johns Hopkins University School of MedicineBarbara K Martin - Johns Hopkins UniversityJ. P Dunn - Johns Hopkins University School of MedicineDavid V Weinberg - Northwestern MedicineJanet L Davis - University of Miami
- Contributors
- Cytomegalovirus Retinitis and Viral Resistance Study Group (Contributor)J Brooks Jackson (Contributor) - University of Iowa, Pathology
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.185(7), pp.861-867
- Publisher
- The University of Chicago Press
- DOI
- 10.1086/339603
- PMID
- 11920309
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 04/01/2002
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984186497302771
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