Comparison of FISH, RFLP and agar dilution methods for testing clarithromycin resistance of Helicobacter pylori

İhsan Hakkı Çiftci; Meltem Uğraş; Gürsel Acartürk; Gülşah Aşık; Birol Şafak; Fatma Hüsniye Dilek

doi:10.5152/tjg.2014.4688

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Comparison of FISH, RFLP and agar dilution methods for testing clarithromycin resistance of Helicobacter pylori

Journal article

Peer reviewed

Comparison of FISH, RFLP and agar dilution methods for testing clarithromycin resistance of Helicobacter pylori

İhsan Hakkı Çiftci, Meltem Uğraş, Gürsel Acartürk, Gülşah Aşık, Birol Şafak and Fatma Hüsniye Dilek

The Turkish journal of gastroenterology, Vol.25 Suppl 1(1), pp.75-80

12/01/2014

DOI: 10.5152/tjg.2014.4688

PMID: 25910373

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Abstract

Clarithromycin resistance is an important factor of Helicobacter pylori (H. pylori) eradication failure in adults and children. There are some tests to determine resistance such as restriction fragment length polimorphism (RFLP), fluorescence in situ hibridisation (FISH), PCR and (culture) agar dilution. Clarithromycin resistance is reported between 16.8%-48.2% in Turkey using PCR, 18% in Japan using RFLP. The aim of the study is to compare the efficacy of FISH, RFLP and culture. Patients with gastric complaint underwent endoscopic examination. H. pylori status of all patients was tested with urea breath test. Gastric biopsy samples obtained from adult patients and children were studied. Each tissue was analised with FISH, PCR-RFLP anda gar dilution. A total 100 patients were positive by UBT and histology for H. pylori. Tissues from 89 adults and 11 children were evaluated. According to FISH and RFLP clarithromycin resistance was 26% and 16% respectively. Among 100 patients H. pylori was cultured in 52 tissue samples, among these samples 7 were resistant to clarithromycin. There was strong correlation between the results of FISH and RFLP; RFLP and culture; and FISH and culture. There is a high ratio of clarithromycin resistance in the studied population. All 3 tests are valuable, but FISH seems to be more sensitive among these tests. We suggest FISH should be used for detecting clarithromycin resistance among H. pylori infected patients before eradication therapy.

Polymerase Chain Reaction

Adolescent

Adult

Aged

Anti-Bacterial Agents - pharmacology

Child

Clarithromycin - pharmacology

Colony Count, Microbial

Drug Resistance, Bacterial

Helicobacter Infections - drug therapy

Helicobacter Infections - microbiology

Helicobacter pylori - drug effects

Humans

In Situ Hybridization, Fluorescence

Microbial Sensitivity Tests - methods

Middle Aged

Polymorphism, Restriction Fragment Length

Young Adult

Details

Title: Subtitle: Comparison of FISH, RFLP and agar dilution methods for testing clarithromycin resistance of Helicobacter pylori
Creators: İhsan Hakkı Çiftci - Sakarya University
Meltem Uğraş
Gürsel Acartürk
Gülşah Aşık
Birol Şafak
Fatma Hüsniye Dilek
Resource Type: Journal article
Publication Details: The Turkish journal of gastroenterology, Vol.25 Suppl 1(1), pp.75-80
DOI: 10.5152/tjg.2014.4688
PMID: 25910373
ISSN: 1300-4948
eISSN: 2148-5607
Language: English
Date published: 12/01/2014
Academic Unit: Stead Family Department of Pediatrics; Gastroenterology, Hepatology, Pancreatology, and Nutrition
Record Identifier: 9985214103602771

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