Journal article
Comparison of In-Situ versus Ex-Situ Delivery of Polyethylenimine-BMP-2 polyplexes for Rat Calvarial Defect Repair via Intraoperative Bioprinting
Biofabrication, Vol.15(1), 015011
01/2023
DOI: 10.1088/1758-5090/ac9f70
PMCID: PMC10012389
PMID: 36322966
Abstract
Gene therapeutic applications combined with bio- and nano-materials have been used to address current shortcomings in bone tissue engineering due to their feasibility, safety and potential capability for clinical translation. Delivery of non-viral vectors can be altered using gene-activated matrices to improve their efficacy to repair bone defects. Ex-situ and in-situ delivery strategies are the most used methods for bone therapy, which have never been directly compared for their potency to repair critical-sized bone defects. In this regard, we first time explore the delivery of polyethylenimine (PEI) complexed plasmid DNA encoding bone morphogenetic protein-2 (PEI-pBMP-2) using the two delivery strategies, ex-situ and in-situ delivery. To realize these gene delivery strategies, we employed intraoperative bioprinting (IOB), enabling us to 3D bioprint bone tissue constructs directly into defect sites in a surgical setting. Here, we demonstrated IOB of an osteogenic bioink loaded with PEI-pBMP-2 for the in-situ delivery approach, and PEI-pBMP-2 transfected rat bone marrow mesenchymal stem cells laden bioink for the ex-situ delivery approach as alternative delivery strategies. We found that in-situ delivery of PEI-pBMP-2 significantly improved bone tissue formation compared to ex-situ delivery. Despite debates amongst individual advantages and disadvantages of ex-situ and in-situ delivery strategies, our results ruled in favor of the in-situ delivery strategy, which could be desirable to use for future clinical applications.
Details
- Title: Subtitle
- Comparison of In-Situ versus Ex-Situ Delivery of Polyethylenimine-BMP-2 polyplexes for Rat Calvarial Defect Repair via Intraoperative Bioprinting
- Creators
- Kazim Kerim Moncal - Penn State, W313 Millennium Science Complex, University Park, Pennsylvania, 16802, UNITED STATES.Miji Yeo - Pennsylvania State UniversityNazmiye T Celik - Pennsylvania State UniversityTimothy Acri - University of IowaElias V Rizk - Penn State, 30 Hope Drive Suite 1200, Entrance B, Hershey, Pennsylvania, 17033, UNITED STATES.Hwabok Wee - Penn State Cancer InstituteGregory S Lewis - Penn State Cancer InstituteAliasger K Salem - University of IowaIbrahim T Ozbolat - Pennsylvania State University
- Resource Type
- Journal article
- Publication Details
- Biofabrication, Vol.15(1), 015011
- DOI
- 10.1088/1758-5090/ac9f70
- PMID
- 36322966
- PMCID
- PMC10012389
- NLM abbreviation
- Biofabrication
- ISSN
- 1758-5082
- eISSN
- 1758-5090
- Grant note
- DOI: 10.13039/501100007619, name: Osteology Foundation, award: 15-042; DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: R01DE028614; DOI: 10.13039/501100011970, name: International Team for Implantology, award: 1275_2017; DOI: 10.13039/100000147, name: Division of Civil, Mechanical and Manufacturing Innovation, award: 1600118
- Language
- English
- Electronic publication date
- 11/02/2022
- Date published
- 01/2023
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9984309751702771
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