Comparison of M.I.C.E. and Etest with CLSI agar dilution for antimicrobial susceptibility testing against oxacillin-resistant Staphylococcus spp

Eloiza H Campana; Cecilia G Carvalhaes; Bruna Nonato; Antonia M de O Machado; Ana C Gales

doi:10.1371/journal.pone.0094627

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Comparison of M.I.C.E. and Etest with CLSI agar dilution for antimicrobial susceptibility testing against oxacillin-resistant Staphylococcus spp

Journal article

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Peer reviewed

Comparison of M.I.C.E. and Etest with CLSI agar dilution for antimicrobial susceptibility testing against oxacillin-resistant Staphylococcus spp

Eloiza H Campana, Cecilia G Carvalhaes, Bruna Nonato, Antonia M de O Machado and Ana C Gales

PloS one, Vol.9(4), pp.e94627-e94627

2014

DOI: 10.1371/journal.pone.0094627

PMCID: PMC3986405

PMID: 24732297

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Abstract

The main objective of this study was to comparatively evaluate the performance of M.I.C.E. and Etest methodologies to that of agar dilution for determining the antimicrobial susceptibility profile of oxacillin-resistant Staphylococcus spp. A total of 100 oxacillin-resistant Staphylococcus spp. isolates were collected from hospitalized patients at a teaching hospital. Antimicrobial susceptibility testing for vancomycin, teicoplanin and linezolid was performed using the reference CLSI agar dilution method (2009), Etest and M.I.C.E. methodologies. The MIC values were interpreted according to CLSI susceptibility breakpoints and compared by regression analysis. In general, the essential agreement (±1-log2) between M.I.C.E. and CLSI agar dilution was 93.0%, 84.0% and 77.0% for linezolid, teicoplanin and vancomycin, respectively. Essential agreement rates between M.I.C.E. and Etest were excellent (>90.0%) for all antibiotics tested. Both strips (M.I.C.E. and Etest) yielded two very major errors for linezolid. Unacceptable minor rates were observed for teicoplanin against CoNS and for vancomycin against S. aureus. According to our results, linezolid and teicoplanin MICs against all staphylococci and S. aureus, respectively, were more accurately predicted by M.I.C.E. strips. However, the Etest showed better performance than M.I.C.E. for predicting vancomycin MICs against all staphylococci. Thus, microbiologists must be aware of the different performance of commercially available gradient strips against staphylococci.

Acetamides - pharmacology

Agar - chemistry

Anti-Infective Agents - pharmacology

Drug Resistance, Bacterial

Humans

Linezolid

Microbial Sensitivity Tests

Oxacillin - pharmacology

Oxazolidinones - pharmacology

Regression Analysis

Reproducibility of Results

Staphylococcus - drug effects

Teicoplanin - pharmacology

Vancomycin - pharmacology

Details

Title: Subtitle: Comparison of M.I.C.E. and Etest with CLSI agar dilution for antimicrobial susceptibility testing against oxacillin-resistant Staphylococcus spp
Creators: Eloiza H Campana - Universidade Federal de São Paulo
Cecilia G Carvalhaes - Universidade Federal de São Paulo
Bruna Nonato - Universidade Federal de São Paulo
Antonia M de O Machado - Hospital São Paulo
Ana C Gales - Universidade Federal de São Paulo
Resource Type: Journal article
Publication Details: PloS one, Vol.9(4), pp.e94627-e94627
DOI: 10.1371/journal.pone.0094627
PMID: 24732297
PMCID: PMC3986405
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science
Language: English
Date published: 2014
Academic Unit: Pathology
Record Identifier: 9984185168302771

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