Journal article
Comparison of Results Obtained with Amplicor HIV-1 DNA PCR Test Version 1.5 Using 100 versus 500 Microliters of Whole Blood
Journal of clinical microbiology, Vol.46(3), pp.1104-1105
03/2008
DOI: 10.1128/JCM.02259-07
PMCID: PMC2268375
PMID: 18199786
Abstract
The Amplicor HIV-1 DNA PCR assay (Roche Diagnostics, Branchburg, NJ) requires 500 μl of whole blood for a diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, and this amount is often difficult to obtain from infants. A comparison was performed using 100 and 500 μl of whole blood from infants less than 18 months of age. The concordance rate for HIV DNA PCR-negative and -positive samples was 100% for the two different volumes.
Details
- Title: Subtitle
- Comparison of Results Obtained with Amplicor HIV-1 DNA PCR Test Version 1.5 Using 100 versus 500 Microliters of Whole Blood
- Creators
- Estelle Piwowar-Manning - Department of Pathology, Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, Maryland 21287Lebah Lugalia - Department of Pathology, Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, Maryland 21287Bosco Kafufu - Department of Pathology, Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, Maryland 21287J. Brooks Jackson - Department of Pathology, Johns Hopkins Medical Institutions, 600 North Wolfe Street, Baltimore, Maryland 21287
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.46(3), pp.1104-1105
- DOI
- 10.1128/JCM.02259-07
- PMID
- 18199786
- PMCID
- PMC2268375
- NLM abbreviation
- J Clin Microbiol
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Publisher
- American Society for Microbiology (ASM)
- Language
- English
- Date published
- 03/2008
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984047668202771
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